About: Structural aspects of antibody-antigen interaction revealed through small random peptide libraries

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Structural aspects of antibody-antigen interaction revealed through small random peptide libraries
Creator	Meloen, Rob Langeveld, Jan Ligtvoet, Gerard Puijk, Wouter Slootstra, Jerry
Source	PMC
abstract	Two small random peptide libraries, one composed of 4550 dodecapeptides and one of 8000 tripeptides, were synthesized in newly developed credit-card format miniPEPSCAN cards (miniPEPSCAN libraries). Each peptide was synthesized in a discrete well (455 peptides/card). The two miniPEPSCAN libraries were screened with three different monoclonal antibodies (Mabs). Two other random peptide libraries, expressed on the wall of bacteria (recombinant libraries) and composed of 10(7) hexa- and octapeptides, were screened with the same three Mabs. The aim of this study was to compare the amino acid sequence of peptides selected from small and large pools of random peptides and, in this way, investigate the potential of small random peptide libraries. The screening of the two miniPEPSCAN libraries resulted in the identification of a surprisingly large number of antibody-binding peptides, while the screening of the large recombinant libraries, using the same Mabs, resulted in the identification of only a small number of peptides. The large number of peptides derived from the small random peptide libraries allowed the determination of consensus sequences. These consensus sequences could be related to small linear and nonlinear parts of the respective epitopes. The small number of peptides derived from the large random peptide libraries could only be related to linear epitopes that were previously mapped using small libraries of overlapping peptides covering the antigenic protein. Thus, with respect to the cost and speed of identifying peptides that resemble linear and nonlinear parts of epitopes, small diversity libraries based on synthetic peptides appear to be superior to large diversity libraries based on expression systems.
has issue date	1996-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01721323
bibo:pmid	9237197
has license	no-cc
sha1sum (hex)	54dadfc8cda67895f4b5890f67e3142556b21e91
schema:url	https://doi.org/10.1007/bf01721323
resource representing a document's title	Structural aspects of antibody-antigen interaction revealed through small random peptide libraries
has PubMed Central identifier	PMC7088776
has PubMed identifier	9237197
schema:publication	Mol Divers
resource representing a document's body	covid:54dadfc8cda67895f4b5890f67e3142556b21e91#body_text
is schema:about of	named entity 'STRUCTURAL' named entity 'RECOMBINANT' named entity 'MONOCLONAL ANTIBODIES' named entity 'CREDIT' named entity 'COMPOSED OF' named entity 'DETERMINATION' named entity 'LINEAR' named entity '107' named entity 'SCREENING' named entity 'EXPRESSED' named entity 'ANTIBODY' named entity 'TO COMPARE' named entity '8000' named entity 'EXPRESSION' named entity 'SUPERIOR' named entity 'ANTIGENIC PROTEIN' named entity 'HEXA' named entity 'ONE OF' named entity 'EPITOPES' named entity 'WALL' named entity 'THESE' covid:arg/54dadfc8cda67895f4b5890f67e3142556b21e91 named entity 'APPEAR' named entity 'NEWLY' named entity 'RANDOM' named entity 'BACTERIA ' named entity 'RELATED' named entity 'PEPTIDES' named entity 'USING' named entity 'LARGE' named entity 'COST' named entity 'NONLINEAR' named entity 'DISCRETE' named entity 'FORMAT' named entity 'SEQUENCES' named entity 'DIFFERENT' named entity 'SYNTHETIC PEPTIDES' named entity 'LIBRARIES' named entity 'NUMBER OF' named entity 'COVERING' named entity 'DERIVED' named entity 'POTENTIAL' named entity 'PEPTIDE' named entity 'ANTIBODY' named entity 'SMALL' named entity 'PEPTIDE LIBRARIES' named entity 'RANDOM' named entity 'REVEALED' named entity 'AIM' named entity 'IDENTIFYING' named entity 'DIVERSITY' named entity 'BINDING' named entity 'COULD BE' named entity 'CONSENSUS' named entity 'PEPTIDE LIBRARIES' named entity 'MAPPED' named entity 'SYNTHESIZED' named entity 'SPEED' named entity 'TRIPEPTIDES' named entity 'ALLOWED' named entity 'ANTIGEN' named entity 'STUDY' named entity 'IDENTIFICATION' named entity 'OVERLAPPING' named entity 'PREVIOUSLY' named entity 'CARD' named entity 'BASED' named entity 'SELECTED' named entity 'POOLS' named entity 'INTERACTION' named entity 'INVESTIGATE' named entity 'SYSTEMS' named entity 'SMALL' named entity 'PARTS' named entity 'RESPECT' named entity 'AMINO ACID SEQUENCE' named entity 'WAY'

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