About: High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus

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About: High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus
Creator	Huang, Ren Zhang, Yu Lian, Yuexiao Wu, Miaoli Zhu, Yujun Huang, Bihong Ma, Lei Xu, Fengjiao Chen, · Li, · Feng Cong, · Guo, ·
topic	covid:54b4d23bdc0456147c7828b1c196a8616e556bf8#this
Source	Medline; PMC
abstract	Rabbits are widely used as models in biological research, and the pathogen status of rabbits used in studies can directly affect the results of experiments. Serological surveillance is the common monitoring method used in laboratory animals. A rapid, sensitive, and cost-effective high-throughput Luminex xMAP assay could be an attractive alternative to labor-intensive enzyme-linked immunosorbent assay (ELISA) methods. In this study, recombinant proteins from rabbit hemorrhagic disease virus and rabbit rotavirus and whole viral lysates of Sendai virus were used as coating antigens in an xMAP assay for the simultaneous detection of antibodies against these pathogens. The xMAP assay showed high specificity, with no cross-reaction with other pathogens. The coefficient of variation for intra-assay and inter-assay comparisons was less than 3% and 4%, respectively, indicating good repeatability and stability of the assay. The xMAP assay exhibited similar limits of detection for rabbit hemorrhagic virus and Sendai virus and was less sensitive for the detection of rabbit rotavirus when compared with commercial ELISA kits. A total of 52 clinical samples were tested simultaneously using both the xMAP assay and ELISA kits. The results obtained using these two methods were 100% coincident. In summary, the novel xMAP assay offers an alternative choice for rapid and sensitive high-throughput detection of antibodies in rabbit serum and can be used as a daily monitoring tool for laboratory animals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-019-04226-9) contains supplementary material, which is available to authorized users.
has issue date	2019-04-15(xsd:dateTime)
bibo:doi	10.1007/s00705-019-04226-9
bibo:pmid	30982935
has license	no-cc
sha1sum (hex)	54b4d23bdc0456147c7828b1c196a8616e556bf8
schema:url	https://doi.org/10.1007/s00705-019-04226-9
resource representing a document's title	High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus
has PubMed Central identifier	PMC7087182
has PubMed identifier	30982935
schema:publication	Arch Virol
resource representing a document's body	covid:54b4d23bdc0456147c7828b1c196a8616e556bf8#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/54b4d23bdc0456147c7828b1c196a8616e556bf8
is schema:about of	named entity 'ASSAY' named entity 'cost-effective' named entity 'monitoring' named entity 'NOVEL' named entity 'LABORATORY ANIMALS' named entity 'CROSS-REACTION' named entity 'MONITORING' named entity 'TOOL' named entity 'COMPARED' named entity '100%' named entity 'RABBIT HEMORRHAGIC DISEASE VIRUS' named entity 'ANTIGENS' named entity 'INTER-' named entity 'USED' named entity 'SENDAI VIRUS' named entity 'THROUGHPUT' named entity 'ATTRACTIVE' named entity 'THROUGHPUT' named entity 'EXPERIMENTS' named entity 'ANTIBODIES' named entity 'STUDY' named entity 'MODELS' named entity 'RAPID' named entity 'DETECTION' named entity 'HIGH' named entity 'SIMULTANEOUS' named entity 'LUMINEX' named entity 'RABBIT' named entity 'DETECTION' named entity 'ROTAVIRUS' named entity 'RABBIT HEMORRHAGIC DISEASE VIRUS' named entity 'SENDAI VIRUS' named entity 'RABBITS' named entity 'GOOD' named entity 'METHODS' named entity 'EFFECTIVE' named entity 'SPECIFICITY' named entity 'SEROLOGICAL' named entity 'DIRECTLY' named entity 'LIMITS OF DETECTION' named entity 'CHOICE' named entity 'LESS THAN' named entity 'CLINICAL' named entity 'AFFECT' named entity 'USING' named entity 'COST' named entity 'ANTIBODIES' named entity 'VIRUS' named entity 'SENSITIVE' named entity 'ELISA' named entity 'DAILY' named entity 'RESULTS' named entity 'RECOMBINANT PROTEINS' named entity 'METHOD' named entity 'STATUS' named entity 'INDICATING' named entity 'RABBIT' named entity 'SIMILAR' named entity 'SIMULTANEOUS' named entity 'ROTAVIRUS' named entity 'TOTAL' named entity 'COEFFICIENT OF VARIATION' named entity 'ASSAY' named entity 'SAMPLES' named entity 'STABILITY' named entity 'SURVEILLANCE' named entity 'STUDIES' named entity 'OBTAINED'

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