About: Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of porcine pegivirus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of porcine pegivirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of porcine pegivirus
Creator	Li, Kai Ye, Yu Wang, Leyi Huang, Dongyan Song, Deping Tang, Yuxin Ding, Zhen Bi, Zhen Gu, Jun Li, Hao Wu, Qiong Lei, Dan Luo, Suoxian
topic	covid:521f53592bc1bead52f709e6b972cc6b5dfdcb23#this
source	Elsevier; Medline; PMC
abstract	Abstract A simple and accurate reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was developed and evaluated for the detection of porcine pegivirus (PPgV). The specific RT-LAMP primers targeting the conserved regions of NS5A genes were designed and used to detect PPgV. The optimal reaction parameter for RT-LAMP assay was 63℃ for 60 min. The detection limit of the RT-LAMP assay was 10 copies of PPgV genome, which was 100 times more sensitive than that of the conventional RT-PCR and comparable to nested RT-PCR and quantitative RT-PCR (qRT-PCR). There was no cross amplification with other related RNA viruses. In the clinical evaluation, the RT-LAMP assay exhibited a similar sensitivity with nested RT-PCR and qRT-PCR. The results indicated that RT-LAMP assay developed in this study could be a highly specific, sensitive, and cost-effective alternative for a rapid detection of PPgV in field settings.
has issue date	2019-08-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2019.04.019
bibo:pmid	31022411
has license	els-covid
sha1sum (hex)	521f53592bc1bead52f709e6b972cc6b5dfdcb23
schema:url	https://doi.org/10.1016/j.jviromet.2019.04.019
resource representing a document's title	Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of porcine pegivirus
has PubMed Central identifier	PMC7113777
has PubMed identifier	31022411
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:521f53592bc1bead52f709e6b972cc6b5dfdcb23#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/521f53592bc1bead52f709e6b972cc6b5dfdcb23
is schema:about of	named entity 'nested' named entity 'cost-effective' named entity 'sensitive' named entity 'evaluated' named entity 'reverse' covid:arg/521f53592bc1bead52f709e6b972cc6b5dfdcb23 named entity 'specific' named entity 'pegivirus' named entity 'min' named entity 'rapid' named entity 'assay' named entity 'sensitive' named entity 'simple' named entity 'reaction' named entity 'qRT-PCR' named entity 'conserved regions' named entity 'nested RT-PCR' named entity 'NS5A' named entity 'qRT-PCR' named entity 'clinical evaluation' named entity 'loop-mediated isothermal amplification' named entity 'pegivirus' named entity 'porcine' named entity 'NS5A' named entity 'pig' named entity 'pathogens' named entity 'GenBank' named entity 'NS3' named entity 'qRT-PCR' named entity 'cDNA' named entity 'Pegivirus' named entity 'detection limit' named entity 'viruses' named entity 'nested RT-PCR' named entity 'epidemiological' named entity 'nested PCR' named entity 'RT-PCR' named entity 'Guangdong Province' named entity 'diagnostic methods' named entity 'agarose gel electrophoresis' named entity 'porcine' named entity 'plasmid' named entity 'vesicular' named entity 'swine farm' named entity 'MgSO' named entity 'gel electrophoresis' named entity 'gene' named entity 'Applied Biosystems' named entity 'semi-quantitative RT-PCR' named entity 'porcine epidemic diarrhea virus' named entity 'RNA' named entity 'Jiangxi' named entity 'PCR' named entity 'epidemiological studies' named entity 'nested RT-PCR' named entity 'denaturation' named entity 'distilled water' named entity 'RT-PCR' named entity 'NS5A' named entity 'RT-PCR' named entity 'China' named entity 'Thermo Scientific' named entity 'qRT-PCR' named entity 'BLAST' named entity 'RT-PCR' named entity 'primers' named entity 'chimpanzees'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software