About: Nicotinic acetylcholine receptors regulate clustering, fusion and acidification of the rat brain synaptic vesicles

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About: Nicotinic acetylcholine receptors regulate clustering, fusion and acidification of the rat brain synaptic vesicles Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Nicotinic acetylcholine receptors regulate clustering, fusion and acidification of the rat brain synaptic vesicles
Creator	Kasatkina, Ludmila Lykhmus, Olena Skok, Maryna Trikash, Irene
Source	Elsevier; Medline; PMC
abstract	The brain nicotinic acetylcholine receptors (nAChRs) expressed in pre-synaptic nerve terminals regulate neurotransmitter release. However, there is no evidence for the expression of nAChRs in synaptic vesicles, which deliver neurotransmitter to synaptic cleft. The aim of this paper was to investigate the presence of nAChRs in synaptic vesicles purified from the rat brain and to study their possible involvement in vesicles life cycle. According to dynamic light scattering analysis, the antibody against extracellular domain (1-208) of α7 nAChR subunit inhibited synaptic vesicles clustering. Sandwich ELISA with nAChR subunit-specific antibodies demonstrated the presence of α4β2, α7 and α7β2nAChR subtypes in synaptic vesicles and showed that α7 and β2 nAChR subunits are co-localized with synaptic vesicle glycoprotein 2A (SV2A). Pre-incubation with either α7-selective agonist PNU282987 or nicotine did not affect synaptic vesicles clustering but delayed their Ca(2+)-dependent fusion with the plasma membranes. In contrast, nicotine but not PNU282987 stimulated acidification of isolated synaptic vesicles, indicating that α4β2 but not α7-containing nAChRs are involved in regulation of proton influx and neurotransmitter refilling. Treatment of rats with levetiracetam, a specific modulator of SV2A, increased the content of α7 nAChRs in synaptic vesicles accompanied by increased clustering but decreased Ca(2+)-dependent fusion. These data for the first time demonstrate the presence of nAChRs in synaptic vesicles and suggest an active involvement of cholinergic regulation in neurotransmitter release. Synaptic vesicles may be an additional target of nicotine inhaled upon smoking and of α7-specific drugs widely discussed as anti-inflammatory and pro-cognitive tools.
has issue date	2020-05-29(xsd:dateTime)
bibo:doi	10.1016/j.neuint.2020.104779
bibo:pmid	32474177
has license	no-cc
sha1sum (hex)	51666299c715b6bbe82f59e978161d0c45b7058d
schema:url	https://doi.org/10.1016/j.neuint.2020.104779
resource representing a document's title	Nicotinic acetylcholine receptors regulate clustering, fusion and acidification of the rat brain synaptic vesicles
has PubMed Central identifier	PMC7256623
has PubMed identifier	32474177
schema:publication	Neurochem Int
resource representing a document's body	covid:51666299c715b6bbe82f59e978161d0c45b7058d#body_text
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