About: Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii

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About: Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii
Creator	Othman, Rofina Chow, Sek Fischer, Rainer Goh, Wai Heng Chua, Kek Lim, Yin Nölke, Greta Schillberg, Stefan Spiegel, Holger Swee, Sherene
source	PMC
abstract	The parasite Toxoplasma gondii causes an opportunistic infection, that is, particularly severe in immunocompromised patients, infants, and neonates. Current antiparasitic drugs are teratogenic and cause hypersensitivity-based toxic side effects especially during prolonged treatment. Furthermore, the recent emergence of drug-resistant toxoplasmosis has reduced the therapeutic impact of such drugs. In an effort to develop recombinant antibodies as a therapeutic alternative, a panel of affinity-matured, T. gondii tachyzoite-specific single-chain variable fragment (scFv) antibodies was selected by phage display and bioinformatic analysis. Further affinity optimization was attempted by introducing point mutations at hotspots within light chain complementarity-determining region 2. This strategy yielded four mutated scFv sequences and a parental scFv that were used to produce five mouse–human chimeric IgGs in Nicotiana benthamiana plants, with yields of 33–72 mg/kg of plant tissue. Immunological analysis confirmed the specific binding of these plant-derived antibodies to T. gondii tachyzoites, and in vitro efficacy was demonstrated by their ability to inhibit the invasion of human fibroblasts and impair parasite infectivity. These novel recombinant antibodies could therefore be suitable for the development of plant-derived immunotherapeutic interventions against toxoplasmosis.
has issue date	2018-12-11(xsd:dateTime)
bibo:doi	10.7717/peerj.5780
bibo:pmid	30581655
has license	cc-by
sha1sum (hex)	50fc9068ff3d31f1caf01c012e7c08b3ea99d985
schema:url	https://doi.org/10.7717/peerj.5780
resource representing a document's title	Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii
has PubMed Central identifier	PMC6294049
has PubMed identifier	30581655
schema:publication	PeerJ
resource representing a document's body	covid:50fc9068ff3d31f1caf01c012e7c08b3ea99d985#body_text
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