About: Mechanism of Nucleic Acid Unwinding by SARS-CoV Helicase

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mechanism of Nucleic Acid Unwinding by SARS-CoV Helicase
Creator	Adedeji, A Marchand, B Snijder, E Te Velthuis, Ajw Weiss, S
Source	Medline; PMC
abstract	The non-structural protein 13 (nsp13) of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) is a helicase that separates double-stranded RNA (dsRNA) or DNA (dsDNA) with a 5′→3′ polarity, using the energy of nucleotide hydrolysis. We determined the minimal mechanism of helicase function by nsp13. We showed a clear unwinding lag with increasing length of the double-stranded region of the nucleic acid, suggesting the presence of intermediates in the unwinding process. To elucidate the nature of the intermediates we carried out transient kinetic analysis of the nsp13 helicase activity. We demonstrated that the enzyme unwinds nucleic acid in discrete steps of 9.3 base-pairs (bp) each, with a catalytic rate of 30 steps per second. Therefore the net unwinding rate is ∼280 base-pairs per second. We also showed that nsp12, the SARS-CoV RNA-dependent RNA polymerase (RdRp), enhances (2-fold) the catalytic efficiency of nsp13 by increasing the step size of nucleic acid (RNA/RNA or DNA/DNA) unwinding. This effect is specific for SARS-CoV nsp12, as no change in nsp13 activity was observed when foot-and-mouth-disease virus RdRp was used in place of nsp12. Our data provide experimental evidence that nsp13 and nsp12 can function in a concerted manner to improve the efficiency of viral replication and enhance our understanding of nsp13 function during SARS-CoV RNA synthesis.
has issue date	2012-05-15(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0036521
bibo:pmid	22615777
has license	cc-by
sha1sum (hex)	50f6c72d60ef3ae2d2235da9a307942a3f6685fe
schema:url	https://doi.org/10.1371/journal.pone.0036521
resource representing a document's title	Mechanism of Nucleic Acid Unwinding by SARS-CoV Helicase
has PubMed Central identifier	PMC3352918
has PubMed identifier	22615777
schema:publication	PLoS One
resource representing a document's body	covid:50f6c72d60ef3ae2d2235da9a307942a3f6685fe#body_text
is schema:about of	named entity 'intermediates' named entity 'RNA' named entity 'experimental' named entity 'acid' named entity 'demonstrated' named entity 'enhance' named entity 'enzyme' named entity 'MINIMAL' named entity 'FOOT' named entity 'MECHANISM' named entity 'DISEASE VIRUS' named entity 'DETERMINED' named entity 'DNA' named entity 'STEP SIZE' named entity 'SARS-COV' named entity 'FUNCTION' named entity 'USED' named entity 'RNA-DEPENDENT RNA POLYMERASE' named entity 'NUCLEOTIDE' named entity 'STRUCTURAL PROTEIN' named entity 'DOUBLE' named entity 'RDRP' named entity 'CATALYTIC RATE' named entity 'OUR' named entity 'VIRAL REPLICATION' named entity 'EFFICIENCY' named entity 'RNA SYNTHESIS' named entity 'PER SECOND' named entity 'NATURE' named entity 'ENERGY' named entity 'IS A' named entity 'MECHANISM' named entity 'HELICASE' named entity 'KINETIC' named entity 'UNDERSTANDING' named entity 'PLACE' named entity 'BASE' named entity 'INCREASING' named entity 'MOUTH' named entity 'DISCRETE' named entity 'HELICASE ACTIVITY' named entity 'PROCESS' named entity 'DATA' named entity 'ANALYSIS' named entity 'NO CHANGE' named entity 'CARRIED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'ENZYME' named entity 'HELICASE' named entity 'NUCLEIC ACID' named entity 'NET' named entity 'CATALYTIC EFFICIENCY' named entity 'EXPERIMENTAL EVIDENCE' named entity 'TRANSIENT' named entity 'PROVIDE' named entity 'CHANGE IN' named entity 'PAIRS' named entity 'LAG' named entity '282' named entity 'CLEAR' named entity 'STEPS' named entity 'NON-' named entity 'FOLD' named entity 'ACTIVITY' named entity 'SPECIFIC' named entity 'EFFECT' named entity 'POLARITY' named entity 'USING' named entity 'REGION' named entity 'OBSERVED' named entity 'PROTEIN ' named entity 'RATE' named entity 'HYDROLYSIS' named entity 'PRESENCE OF' named entity 'INTERMEDIATES' named entity 'SARS-COV' named entity 'INCREASING LENGTH'

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