About: Structure-efficacy relationships of immunostimulatory activity of CpG-containing oligodeoxynucleotides on mouse spleen cells

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About: Structure-efficacy relationships of immunostimulatory activity of CpG-containing oligodeoxynucleotides on mouse spleen cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Structure-efficacy relationships of immunostimulatory activity of CpG-containing oligodeoxynucleotides on mouse spleen cells
Creator	Li, Na Sun, Xiao Du, Hai-Yan Ji, Ai-Guo Ou, Lun Shang, Ming-Mei Song, Hai-Feng Tang, Zhong-Ming Wang, Qing-Qing Xia, Shao-You Zou, Jia
source	PMC
abstract	AIM: To study the relationship between primary structures of oligodeoxynucleo-tides (ODN) containing unmethylated deoxycytidyl-deoxyguanosine (CpG) dinucleotide motifs and their immunostimulatory activities in mouse spleen cells. METHODS: A series of CpG ODN with different primary structures were synthesized. Their capabilities to stimulate mouse spleen cell proliferation were determined by [(3)H]thymidine incorporation assay. Cytokine (interleukin [IL]-6, IL-12, and IFN-α) secretion spectra induced by CpG ODN were assessed by ELISA. The ability of CpG ODN to activate natural killer cells was evaluated by standard 4 h (51)Cr-release assay. Flow cytometry was utilized to examine the expressions of various lymphocyte surface molecules on diverse immunocytes. An effective CpG ODN for murine, ODN1826, was set as the template of modification and the positive control. RESULTS: The immunostimulatory activities of CpG ODN with different sequences and compositions varied markedly, both in character and in extent. It was useless for improving the immunostimulatory activity of ODN1826 by simply increasing the functional hexameric CpG motif number, modifying the site of CpG motifs, or changing the distance between multi-CpG motifs. However, an addition of a self-complementary palindrome structure at the 3′-end, but not the 5′-end of CpG ODN, aroused marked improvement in its activity. Several designed ODN had superior comprehensive immunostimulatory properties compared to ODN1826. CONCLUSION: The immunostimulatory activity of a CpG ODN was relevant to its primary structure. It was useless for promoting immunostimulatory activity to simply change CpG motif number, space, or distance. The 3′-end palindrome structure of CpG ODN is associated with enhanced immunostimulatory activity.
has issue date	2007-10-01(xsd:dateTime)
bibo:doi	10.1111/j.1745-7254.2007.00628.x
bibo:pmid	17883951
has license	no-cc
sha1sum (hex)	50f39ca6a188a1df262079682ec228ddd45307df
schema:url	https://doi.org/10.1111/j.1745-7254.2007.00628.x
resource representing a document's title	Structure-efficacy relationships of immunostimulatory activity of CpG-containing oligodeoxynucleotides on mouse spleen cells
has PubMed Central identifier	PMC7091584
has PubMed identifier	17883951
schema:publication	Acta Pharmacol Sin
resource representing a document's body	covid:50f39ca6a188a1df262079682ec228ddd45307df#body_text
is schema:about of	named entity 'CpG' named entity 'assay' named entity 'useless' named entity 'changing' named entity 'change' named entity 'palindrome' named entity 'Methods' named entity 'cells' named entity 'site' named entity 'ODN' named entity 'SYNTHESIZED' named entity 'ODN' named entity 'surface' named entity 'The' named entity 'spleen' named entity 'activity' named entity 'CpG' named entity 'positive control' named entity 'assessed' named entity 'Cytokine' named entity 'murine' named entity 'improvement' named entity 'distance' named entity 'thymidine' named entity 'distance' named entity 'primary structure' named entity 'CpG ODN' named entity 'CpG' named entity 'CpG' named entity 'CpG ODN' named entity 'secretion' named entity 'CpG ODN' named entity 'CpG ODN' named entity '5'-end' named entity 'CpG ODN' named entity 'CpG ODN' named entity 'hexameric' named entity 'palindrome' named entity 'immunostimulatory' named entity 'ODN' named entity 'immunostimulatory' named entity 'compartmentalization' named entity 'Boston' named entity 'palindrome' named entity 'IL-12' named entity 'CpG ODN' named entity 'motif' named entity 'Canada' named entity '3'-end' named entity 'ODN' named entity 'CD19' named entity 'hexameric' named entity 'cell-surface molecules' named entity 'CpG motifs' named entity 'pDC' named entity '5'-end' named entity 'CpG ODN' named entity 'µmol/L' named entity 'SAR' named entity 'CpG ODN' named entity 'CpG ODN' named entity 'IL-12' named entity 'immunostimulatory' named entity 'pathogen-associated molecular patterns' named entity 'effector cells' named entity 'intracellular' named entity 'antibody' named entity 'dimeric' named entity 'adaptive immune responses' named entity 'CpG ODN' named entity 'hexameric'

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