About: Role of plasmid carrying bla(NDM) in mediating antibiotic resistance among Acinetobacter baumannii clinical isolates from Egypt

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About: Role of plasmid carrying bla(NDM) in mediating antibiotic resistance among Acinetobacter baumannii clinical isolates from Egypt Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Role of plasmid carrying bla(NDM) in mediating antibiotic resistance among Acinetobacter baumannii clinical isolates from Egypt
Creator	Abouelfetouh, Alaa Aboulmagd, · Torky, Aisha
Source	PMC
abstract	We investigated antibiotic resistance levels among bla(NDM)-positive (n = 9) and -negative (n = 65) A. baumannii clinical isolates collected in 2010 and 2015 from Alexandria Main University Hospital, Egypt using disc diffusion and minimum inhibitory concentration (MIC) determination. Plasmids from bla(NDM)-positive isolates were transformed into a carbapenem-susceptible A. baumannii (CS-AB) isolate to assess the role of plasmid transfer in mediating carbapenem resistance. Imipenem, meropenem, and ertapenem MIC90 values against bla(NDM)-positive isolates were 128, > 256, and 256 µg/mL, respectively. Plasmid isolation and polymerase chain reaction revealed that bla(NDM) was plasmid mediated. The plasmids were electroporated into the cells of a CS-AB isolate at an efficiency of 1.3 × 10(–8) to 2.6 × 10(–7), transforming them to bla(NDM)-positive carbapenem-resistant cells with an imipenem MIC increase of 256-fold. In addition to carbapenem resistance, the bla(NDM)-positive isolates also exhibited higher levels of cephalosporins, tetracycline, aminoglycosides, fluoroquinolones, and colistin resistance than the bla(NDM)-negative isolates. Acquisition of bla(NDM)-carrying plasmids dramatically increased imipenem resistance among A. baumannii isolates. Intriguingly, bla(NDM)-positive isolates also showed a high degree of resistance to antibiotics of different classes. The potential co-existence of different resistance determinants on A. baumannii plasmids and their possible transfer owing to the natural competence of the pathogen are especially alarming. More effective infection control and antibiotic stewardship programs are needed to curb the spread and treat such infections in both hospital and community settings. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13205-020-2157-y) contains supplementary material, which is available to authorized users.
has issue date	2020-03-16(xsd:dateTime)
bibo:doi	10.1007/s13205-020-2157-y
bibo:pmid	32206504
has license	no-cc
sha1sum (hex)	4f987ba03135ab3298f30e8f331b99fddd97dfb5
schema:url	https://doi.org/10.1007/s13205-020-2157-y
resource representing a document's title	Role of plasmid carrying bla(NDM) in mediating antibiotic resistance among Acinetobacter baumannii clinical isolates from Egypt
has PubMed Central identifier	PMC7076099
has PubMed identifier	32206504
schema:publication	3 Biotech
resource representing a document's body	covid:4f987ba03135ab3298f30e8f331b99fddd97dfb5#body_text
is schema:about of	named entity 'efficiency' named entity 'resistance' named entity 'programs' named entity 'isolates' named entity 'bla' named entity 'imipenem' named entity 'mediating' named entity 'resistance' named entity 'disc' named entity 'isolates' named entity 'ISOLATE' named entity 'ANTIBIOTIC' named entity 'MIC' named entity 'CEPHALOSPORINS' named entity 'DIFFERENT' named entity 'CARBAPENEM' named entity 'ALARMING' named entity 'DISC' named entity 'POLYMERASE CHAIN REACTION' named entity 'NEEDED' named entity 'ISOLATES' named entity 'MEDIATING' named entity 'levels' named entity 'isolates' named entity 'bla' named entity 'resistance' named entity 'isolates' named entity 'cephalosporins' named entity 'isolate' named entity 'fold' named entity 'bla' named entity 'negative' named entity 'diffusion' named entity 'antibiotic resistance' named entity 'MIC' named entity 'plasmids' named entity 'meropenem' named entity 'A. baumannii' named entity 'plasmid' named entity 'tetracycline' named entity 'plasmids' named entity 'A. baumannii' named entity 'colistin' named entity 'antibiotic resistance' named entity 'plasmid' named entity 'imipenem' named entity 'Acinetobacter' named entity 'Kuwait' named entity 'tazobactam' named entity 'plasmid' named entity 'plasmid' named entity 'doxycycline' named entity 'MIC' named entity 'imipenem' named entity 'Turkey' named entity 'highly conserved' named entity 'ertapenem' named entity 'ertapenem' named entity 'carbapenem' named entity 'piperacillin' named entity 'second-line treatment' named entity 'β-lactams' named entity 'plasmid' named entity 'imipenem' named entity 'ciprofloxacin' named entity 'A. baumannii' named entity 'Sigma Aldrich' named entity 'aminoglycoside' named entity 'PCR amplification' named entity 'plasmids' named entity 'DNA ladder' named entity 'diffusion' named entity 'ATCC' named entity 'carbapenem' named entity 'PCR' named entity 'trimethoprim' named entity 'Toleman' named entity 'A. baumannii' named entity 'Thermo Scientific'

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