About: Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone

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About: Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone
Creator	Perez, Andres Yuan, Jing Li, Huan Liu, Wei Li, Puyuan Zhao, Xiangna Li, H Dong, Derong Li, Lin Liu, Chao Cui, Lifei Hu, Xuan Li, Boxing Li, Erna Lin, Weishi Ma, Yanyan Wang, Xuesong Wei, Xiao Li, Hu Li, E
Source	Medline; PMC
abstract	Ebola virus (EBOV) can lead to severe hemorrhagic fever with a high risk of death in humans and other primates. To guide treatment and prevent spread of the viral infection, a rapid and sensitive detection method is required for clinical samples. Here, we described and evaluated a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method to detect Zaire ebolavirus using the nucleoprotein gene (NP) as a target sequence. Two different techniques were used, a calcein/Mn(2+) complex chromogenic method and real-time turbidity monitoring. The RT-LAMP assay detected the NP target sequence with a limit of 4.56 copies/μL within 45 min under 61°C, a similar even or increase in sensitivity than that of real-time reverse transcription-polymerase chain reaction (RT-PCR). Additionally, all pseudoviral particles or non- Zaire EBOV genomes were negative for LAMP detection, indicating that the assay was highly specific for EBOV. To appraise the availability of the RT-LAMP method for use in clinical diagnosis of EBOV, of 417 blood or swab samples collected from patients with clinically suspected infections in Sierra Leone, 307 were identified for RT-LAMP-based surveillance of EBOV. Therefore, the highly specific and sensitive RT-LAMP method allows the rapid detection of EBOV, and is a suitable tool for clinical screening, diagnosis, and primary quarantine purposes.
has issue date	2015-12-01(xsd:dateTime)
bibo:doi	10.3389/fmicb.2015.01332
bibo:pmid	26648918
has license	cc-by
sha1sum (hex)	4ecd138ab31f520b06c5cf095657fab2685a8209
schema:url	https://doi.org/10.3389/fmicb.2015.01332
resource representing a document's title	Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone
has PubMed Central identifier	PMC4664619
has PubMed identifier	26648918
schema:publication	Front Microbiol
resource representing a document's body	covid:4ecd138ab31f520b06c5cf095657fab2685a8209#body_text
is schema:about of	named entity 'min' named entity 'sequence' named entity 'rapid' named entity 'EBOV' named entity 'nucleoprotein' named entity 'REVERSE TRANSCRIPTION' named entity 'INFECTIONS' named entity 'SURVEILLANCE' named entity 'SPECIFIC' named entity 'ASSAY' named entity 'HIGHLY' named entity 'DETECTION' named entity 'RAPID' named entity 'SUITABLE' named entity 'TARGETING' named entity 'DETECTION METHOD' named entity 'SWAB' named entity 'SENSITIVITY' named entity 'TO DETECT' named entity '80%' named entity 'SPREAD' named entity 'ZAIRE' named entity 'DESCRIBED' named entity 'METHOD' named entity 'USING' named entity 'COPIES' named entity 'LAMP' named entity 'SIERRA LEONE' named entity 'HIGH RISK OF' named entity 'SEVERE' named entity 'PATIENTS' named entity 'VISUAL' named entity 'CLINICAL' named entity 'TREATMENT' named entity 'PCR' named entity 'SEQUENCE' named entity 'LIMIT' named entity 'LEAD' named entity 'MONITORING' named entity 'PARTICLES' named entity 'BLOOD' named entity 'NUCLEOPROTEIN' named entity 'GENE' named entity 'ZAIRE EBOLAVIRUS' named entity 'SURVEY' named entity 'SAMPLES' named entity 'SAMPLES' named entity 'POLYMERASE CHAIN REACTION' named entity 'TOOL' named entity 'NON-' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'PRIMARY' named entity 'BASED' named entity 'TURBIDITY' named entity 'GENOMES' named entity 'NUCLEOPROTEIN' named entity 'USE' named entity 'HUMANS' named entity 'PREVENT' named entity 'DIFFERENT' named entity 'INDICATING' named entity 'CLINICAL DIAGNOSIS'

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