About: TRIM28 regulates SARS-CoV-2 cell entry by targeting ACE2

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	TRIM28 regulates SARS-CoV-2 cell entry by targeting ACE2
Creator	Wang, Ying Zhang, Peng Du, Tao Fan, Yingzhe Huang, Dekui Huang, Yitong Liu, Zongjun Wang, Nanping Wang, Yinfang
Source	BioRxiv
abstract	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease 2019 (COVID-19), it binds to angiotensin-converting enzyme 2 (ACE2) to enter into human cells. The expression level of ACE2 potentially determine the susceptibility and severity of COVID-19, it is thus of importance to understand the regulatory mechanism of ACE2 expression. Tripartite motif containing 28 (TRIM28) is known to be involved in multiple processes including antiviral restriction, endogenous retrovirus latency and immune response, it is recently reported to be co-expressed with SARS-CoV-2 receptor in type II pneumocytes; however, the roles of TRIM28 in ACE2 expression and SARS-CoV-2 cell entry remain unclear. This study showed that knockdown of TRIM28 induces ACE2 expression and increases pseudotyped SARS-CoV-2 cell entry of A549 cells and primary pulmonary alveolar epithelial cells (PAEpiCs). In a co-culture model of NK cells and lung epithelial cells, our results demonstrated that NK cells inhibit TRIM28 and promote ACE2 expression in lung epithelial cells, which was partially reversed by depletion of interleukin-2 and blocking of granzyme B in the co-culture medium. Furthermore, TRIM28 knockdown enhanced interferon-γ (IFN-γ)-induced ACE2 expression through a mechanism involving upregulating IFN-γ receptor 2 (IFNGR2) in both A549 and PAEpiCs. Importantly, the upregulated ACE2 induced by TRIM28 knockdown and co-culture of NK cells was partially reversed by dexamethasone in A549 cells but not PAEpiCs. Our study identified TRIM28 as a novel regulator of ACE2 expression and SARS-CoV-2 cell entry.
has issue date	2020-08-12(xsd:dateTime)
bibo:doi	10.1101/2020.08.12.247825
has license	biorxiv
sha1sum (hex)	4e49bb1cf0d7915256efd156930bb15f63e65a88
schema:url	https://doi.org/10.1101/2020.08.12.247825
resource representing a document's title	TRIM28 regulates SARS-CoV-2 cell entry by targeting ACE2
schema:publication	bioRxiv
resource representing a document's body	covid:4e49bb1cf0d7915256efd156930bb15f63e65a88#body_text
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