About: A Fluorometric Method of Measuring Carboxypeptidase Activities for Angiotensin II and Apelin-13

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About: A Fluorometric Method of Measuring Carboxypeptidase Activities for Angiotensin II and Apelin-13 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Fluorometric Method of Measuring Carboxypeptidase Activities for Angiotensin II and Apelin-13
Creator	Jin, Jing Batlle, Daniel Wysocki, Jan Ye, Minghao Liu, Pan Serfozo, Peter Souma, Tomokazu
Source	PMC
abstract	Degradation of the biologically potent octapeptide angiotensin Ang II-(1-8) is mediated by the activities of several peptidases. The conversion of Ang II to the septapeptide Ang-(1-7) is of particular interest as the latter also confers organ protection. The conversion is catalyzed by angiotensin-converting enzyme 2 and other enzymes that selectively cleave the peptide bond between the proline and the phenylalanine at the carboxyl terminus of Ang II. The contribution of various enzyme activities that collectively lead to the formation of Ang-(1-7) from Ang II, in both normal conditions and in disease states, remains only partially understood. This is largely due to the lack of a reliable and sensitive method to detect these converting activities in complex samples, such as blood and tissues. Here, we report a fluorometric method to measure carboxypeptidase activities that cleave the proline-phenylalanine dipeptide bond in Ang II. This method is also suitable for measuring the conversion of apelin-13. The assay detects the release of phenylalanine amino acid in a reaction with the yeast enzyme of phenylalanine ammonia lyase (PAL). When used in cell and mouse organs, the assay can robustly measure endogenous Ang II and apelin-13-converting activities involved in the renin-angiotensin and the apelinergic systems, respectively.
has issue date	2017-04-05(xsd:dateTime)
bibo:doi	10.1038/srep45473
bibo:pmid	28378780
has license	cc-by
sha1sum (hex)	4e2f13bbb7637dc94eba63f2170cbcbceafcc4c0
schema:url	https://doi.org/10.1038/srep45473
resource representing a document's title	A Fluorometric Method of Measuring Carboxypeptidase Activities for Angiotensin II and Apelin-13
has PubMed Central identifier	PMC5381230
has PubMed identifier	28378780
schema:publication	Sci Rep
resource representing a document's body	covid:4e2f13bbb7637dc94eba63f2170cbcbceafcc4c0#body_text
is schema:about of	named entity 'selectively' named entity 'Apelin' named entity 'ANGIOTENSIN II' named entity 'BOND' named entity 'CONVERTING' named entity 'MEASURING' named entity 'DISEASE' named entity 'ENDOGENOUS' named entity 'TERMINUS' named entity 'RELIABLE' named entity 'PHENYLALANINE' named entity 'SAMPLES' named entity 'CONDITIONS' named entity 'USED' named entity 'PEPTIDE BOND' named entity 'FORMATION' named entity 'ASSAY' named entity 'PROLINE' named entity 'REACTION' named entity 'RELEASE' named entity 'ANGIOTENSIN-CONVERTING ENZYME 2' named entity 'SYSTEMS' named entity 'ENZYMES' named entity 'METHOD' named entity 'SENSITIVE' named entity 'METHOD' named entity 'AMMONIA LYASE' named entity 'INVOLVED' named entity 'THESE' named entity 'COMPLEX' covid:arg/4e2f13bbb7637dc94eba63f2170cbcbceafcc4c0 named entity 'APELIN-13' named entity 'PARTIALLY' named entity 'APELIN-13' named entity 'MEASURING' named entity 'CARBOXYPEPTIDASE' named entity 'REPORT' named entity 'ANG' named entity 'MEASURE' named entity 'ENZYME' named entity 'ORGAN PROTECTION' named entity 'DIPEPTIDE' named entity 'HERE' named entity 'CONTRIBUTION' named entity 'NORMAL' named entity 'CONVERSION' named entity 'UNDERSTOOD' named entity 'DUE TO' named entity 'STATES' named entity 'MOUSE' named entity 'CARBOXYPEPTIDASE' named entity 'AMINO ACID' named entity 'SUITABLE' named entity 'ACTIVITIES' named entity 'LACK' named entity 'ORGANS' named entity 'TO DETECT' named entity 'CELL' named entity 'VARIOUS' named entity 'ANG II' named entity 'YEAST' named entity 'CARBOXYL' named entity 'ACTIVITIES' named entity 'LEAD' named entity 'BLOOD' named entity 'TISSUES' named entity 'TO MEASURE' named entity 'LARGELY' named entity 'carboxyl terminus' named entity 'endogenous' named entity 'fluorometric' named entity 'cell' named entity 'disease' named entity 'detects' named entity 'activities'

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