About: Biological membranes are continuously remodeled in the cell by specific membrane-shaping machineries to form, for example, tubes and vesicles. We examine fundamental mechanisms involved in the vesiculation processes induced by a cluster of envelope (E) and membrane (M) proteins of the dengue virus (DENV) using molecular dynamics simulations and a coarse-grained model. We show that an arrangement of three E-M heterotetramers (EM(3)) works as a bending unit and an ordered cluster of five such units generates a closed vesicle, reminiscent of the virus budding process. In silico mutagenesis of two charged residues of the anchor helices of the envelope proteins of DENV shows that Arg-471 and Arg-60 are fundamental to produce bending stress on the membrane. The fine-tuning between the size of the EM(3) unit and its specific bending action suggests this protein unit is an important factor in determining the viral particle size.

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About: Biological membranes are continuously remodeled in the cell by specific membrane-shaping machineries to form, for example, tubes and vesicles. We examine fundamental mechanisms involved in the vesiculation processes induced by a cluster of envelope (E) and membrane (M) proteins of the dengue virus (DENV) using molecular dynamics simulations and a coarse-grained model. We show that an arrangement of three E-M heterotetramers (EM(3)) works as a bending unit and an ordered cluster of five such units generates a closed vesicle, reminiscent of the virus budding process. In silico mutagenesis of two charged residues of the anchor helices of the envelope proteins of DENV shows that Arg-471 and Arg-60 are fundamental to produce bending stress on the membrane. The fine-tuning between the size of the EM(3) unit and its specific bending action suggests this protein unit is an important factor in determining the viral particle size. Goto Sponge NotDistinct Permalink

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type	abstract
value	Biological membranes are continuously remodeled in the cell by specific membrane-shaping machineries to form, for example, tubes and vesicles. We examine fundamental mechanisms involved in the vesiculation processes induced by a cluster of envelope (E) and membrane (M) proteins of the dengue virus (DENV) using molecular dynamics simulations and a coarse-grained model. We show that an arrangement of three E-M heterotetramers (EM(3)) works as a bending unit and an ordered cluster of five such units generates a closed vesicle, reminiscent of the virus budding process. In silico mutagenesis of two charged residues of the anchor helices of the envelope proteins of DENV shows that Arg-471 and Arg-60 are fundamental to produce bending stress on the membrane. The fine-tuning between the size of the EM(3) unit and its specific bending action suggests this protein unit is an important factor in determining the viral particle size.
subject	Virology Flaviviruses Dengue fever Glucogenic amino acids Membrane biology
part of	Membrane vesiculation induced by proteins of the dengue virus envelope studied by molecular dynamics simulations
is abstract of	Membrane vesiculation induced by proteins of the dengue virus envelope studied by molecular dynamics simulations
is hasSource of	covid:ann/target/bf747d98be87f66d4e8bf6962c6be6c756d8a021 covid:ann/target/0562c5329f5e13b5acb64338d13ce3c79bb650b4 covid:ann/target/f79f810ded8424cb9fb569308810c990ff59d65a covid:ann/target/f4831622288ea7215de5a56a6800e565652dc6c4 covid:ann/target/83dce75d33b4a47cbb4029d2ce7bb380c8fd4c82 »more»

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