About: Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus
Creator	Chen, Keyan Gao, Feng Gao, Wei He, Wenqi Song, Deguang Zhao, Kui Wang, Chengli Zhao, Chuanbo
source	Medline; PMC
abstract	BACKGROUND: The incidence of PHE among pigs in many countries is on the rise, and it has caused great economic losses to the pig industry. Therefore, the development of a sensitive, specific, and easily-performed assay is crucial for the rapid detection and surveillance of PHE-CoV infection and transmission. RESULTS: An immunochromatographic strip was developed for the detection of PHE-CoV. The colloidal gold-labeled MAb 4D4 was used as the detection reagent, and the MAb 1E2 and goat anti-mouse IgG coated the strip's test and control lines, respectively. The immunochromatographic strip was capable of specifically detecting PHE-CoV with a HA unit of 2 within 10 min. Storage of the strips at room temperature for 6 months or at 4°C for 12 months did not change their sensitivity or specificity. Using RT-PCR as a reference test, the relative specificity and sensitivity of the immunochromatographic strip were determined to be 100% and 97.78%, respectively. There was an excellent agreement between the results obtained by RT-PCR and the immunochromatographic strips (kappa = 0.976). Additionally, there was a strong agreement between the sandwich enzyme-linked immunosorbent assay (ELISA) and immunochromatographic strips (Kappa = 0.976). When the immunochromatographic strips were used for diagnosing PHE-CoV infection in the Jilin Province, the PHE-CoV-positive rate ranged from 61.54% in the Jilin district to 17.95% in the Songyuan district. CONCLUSIONS: Based on its high specificity, sensitivity, and stability, the immunochromatographic strip would be suitable for on-site detection of PHE-CoV for surveillance and epidemiological purposes.
has issue date	2012-08-24(xsd:dateTime)
bibo:doi	10.1186/1743-422x-9-172
bibo:pmid	22920192
has license	cc-by
sha1sum (hex)	4d4de01a6f9ad02ac0939d54713c209153fa66df
schema:url	https://doi.org/10.1186/1743-422x-9-172
resource representing a document's title	Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus
has PubMed Central identifier	PMC3508965
has PubMed identifier	22920192
schema:publication	Virol J
resource representing a document's body	covid:4d4de01a6f9ad02ac0939d54713c209153fa66df#body_text
is schema:about of	named entity 'RAPID' named entity 'PHE-' named entity 'EVALUATION' named entity 'INFECTION' named entity 'SPECIFIC' named entity 'ASSAY' named entity 'STRIP' named entity 'SURVEILLANCE' named entity 'EASILY' named entity 'INDUSTRY' named entity 'SENSITIVE' named entity 'TRANSMISSION' named entity 'ECONOMIC' named entity 'COUNTRIES' named entity 'BACKGROUND' named entity 'CAUSED' named entity 'DETECTION' named entity 'PIG' named entity 'RAPID' named entity 'PIGS' named entity 'INCIDENCE' named entity 'PHE' named entity 'PERFORMED' named entity 'DEVELOPMENT' named entity 'PORCINE HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS' named entity 'DETECTION' named entity 'RISE' named entity 'GREAT' named entity 'DEVELOPMENT' covid:arg/4d4de01a6f9ad02ac0939d54713c209153fa66df named entity 'PHE' named entity 'pig' named entity 'PHE' named entity 'immunochromatographic' named entity 'chromatography' named entity 'antibody' named entity 'MAb' named entity 'antibody' named entity 'HCV' named entity 'MAb' named entity 'nitrocellulose' named entity 'immunochromatographic' named entity 'vomiting' named entity 'colloidal gold' named entity 'colloidal gold' named entity 'clinical diagnosis' named entity 'immunochromatographic' named entity 'correlation' named entity 'colloidal gold' named entity 'colloidal gold' named entity 'mononuclear cells' named entity 'Colloidal gold' named entity 'medical treatment' named entity 'transmission electron microscope' named entity 'immunochromatographic' named entity 'Liaoyuan' named entity 'viruses' named entity 'RT-PCR' named entity 'IgG' named entity 'sample pad' named entity 'virus' named entity 'subcutaneously' named entity 'colloidal gold' named entity 'hybridoma cells' named entity 'antibodies' named entity 'w/v' named entity 'neurotropic' named entity 'Colloidal gold' named entity 'Freund's complete adjuvant' named entity 'immunohistochemistry' named entity 'Enzyme-linked immunosorbent assay' named entity 'pig farms' named entity 'mortality rate' named entity 'infection'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software