About: Molecular signatures of epithelial oviduct cells of a laying hen (Gallus gallus domesticus) and quail (Coturnix japonica)

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About: Molecular signatures of epithelial oviduct cells of a laying hen (Gallus gallus domesticus) and quail (Coturnix japonica) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular signatures of epithelial oviduct cells of a laying hen (Gallus gallus domesticus) and quail (Coturnix japonica)
Creator	Pain, Bertrand Bednarczyk, Marek Dunisławska, Aleksandra Stadnicka, Katarzyna Sławińska, Anna
Source	Medline; PMC
abstract	BACKGROUND: In this work we have determined molecular signatures of oviduct epithelial and progenitor cells. We have proposed a panel of selected marker genes, which correspond with the phenotype of oviduct cells of a laying hen (Gallus gallus domesticus) and quail (Coturnix japonica). We demonstrated differences in characteristics of those cells, in tissue and in vitro, with respect to different anatomical and functional parts of the oviduct (infundibulum (INF), distal magnum (DM, and proximal magnum (PM)). The following gene expression signatures were studied: (1) oviduct markers (estrogen receptor 1, ovalbumin, and SPINK7 - ovomucoid), (2) epithelial markers (keratin 5, keratin 14, and occludin) and (3) stem-like/progenitor markers (CD44 glycoprotein, LGR5, Musashi-1, and sex determining region Y-box 9, Nanog homebox, OCT4/cPOUV gene encoding transcription factor POU5F3). RESULTS: In chicken, the expression of oviduct markers increased toward the proximal oviduct. Epithelial markers keratin14 and occludin were high in distal oviduct and decreased toward the proximal magnum. In quail oviduct tissue, the gene expression pattern of oviduct/epithelial markers was similar to chicken. The markers of progenitors/stemness in hen oviduct (Musashi-1 and CD44 glycoprotein) had the highest relative expression in the infundibulum and decreased toward the proximal magnum. In quail, we found significant expression of four progenitor markers (LGR5 gene, SRY sex determining region Y-box 9, OCT4/cPOUV gene, and CD44 glycoprotein) that were largely present in the distal oviduct. After in vitro culture of oviduct cells, the gene expression pattern has changed. High secretive potential of magnum-derived cells diminished by using decreased abundance of mRNA. On the other hand, chicken oviduct cells originating from the infundibulum gained ability to express OVM and OVAL. Epithelial character of the cells was maintained in vitro. Among progenitor markers, both hen and quail cells expressed high level of SOX9, LGR5 and Musashi-1. CONCLUSION: Analysis of tissue material revealed gradual increase/decrease pattern in majority of the oviduct markers in both species. This pattern changed after the oviductal cells have been cultured in vitro. The results can provide molecular tools to validate the phenotype of in vitro biological models from reproductive tissue. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12861-018-0168-2) contains supplementary material, which is available to authorized users.
has issue date	2018-04-04(xsd:dateTime)
bibo:doi	10.1186/s12861-018-0168-2
bibo:pmid	29614966
has license	cc-by
sha1sum (hex)	4a9bdf497f276c36abac9da179a0bb1488617a40
schema:url	https://doi.org/10.1186/s12861-018-0168-2
resource representing a document's title	Molecular signatures of epithelial oviduct cells of a laying hen (Gallus gallus domesticus) and quail (Coturnix japonica)
has PubMed Central identifier	PMC5883888
has PubMed identifier	29614966
schema:publication	BMC Dev Biol
resource representing a document's body	covid:4a9bdf497f276c36abac9da179a0bb1488617a40#body_text
is schema:about of	named entity 'SELECTED' named entity 'PROGENITOR CELLS' named entity 'MOLECULAR' named entity 'PARTS' named entity 'EPITHELIAL' named entity 'SIGNATURES' named entity 'distal' named entity 'oviduct' named entity 'gene expression' named entity 'phenotype' named entity 'Coturnix japonica' named entity 'quail' named entity 'Poland' named entity 'fimbria' named entity 'muscle' named entity 'progenitor cells' named entity 'reference genes' named entity 'cell culture' named entity 'distal' named entity 'basal' named entity 'quail' named entity 'oviduct' named entity 'epithelial' named entity 'Campylobacter' named entity 'epithelium' named entity 'Vilnius, Lithuania' named entity 'SOX9' named entity 'ESR1' named entity 'gene' named entity 'cell types' named entity 'Poland' named entity 'quail' named entity 'epithelial cell' named entity 'Gdansk' named entity 'quail' named entity 'bioreactors' named entity 'Warszawa' named entity 'cell lines' named entity 'oviduct' named entity 'gene expression' named entity 'epithelial' named entity 'quail' named entity 'collagenase' named entity 'epithelial cells' named entity 'LGR5' named entity 'distal' named entity 'oviduct' named entity 'oviduct' named entity 'OVM' named entity 'quail' named entity 'oviduct' named entity 'oviduct' named entity 'oviduct' named entity 'ESR1' named entity 'MSI1' named entity 'Poznan' named entity 'Student's t-test' named entity 'fluorescence' named entity 'ovalbumin' named entity 'mRNA' named entity 'fibroblast' named entity 'cancer stem cell' named entity 'morphological' named entity 'infundibulum' named entity 'polystyrene' named entity 'quail' named entity 'stemness' named entity 'Strong induction' named entity 'fallopian tube' named entity 'oviduct' named entity 'proximal' named entity 'CD44' named entity 'Motile cilia' named entity 'ACTB' named entity 'Cary, NC' named entity 'epithelial' named entity 'RNA'

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