About: Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay

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About: Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay
Creator	Ki, Chang-Seok Kim, Suk Lee, Nam
Source	Elsevier; Medline; PMC
abstract	Acute viral respiratory infections are among the most common causes of human disease. Rapid and accurate diagnosis of viral respiratory infections is important for providing timely therapeutic interventions. This study evaluated a new multiplex PCR assay (Seegene Inc., Seoul, Korea) for simultaneous detection and identification of 12 respiratory viruses using two primer mixes. The viruses included parainfluenza viruses 1, 2, and 3, human metapneumovirus, human coronavirus 229E/NL63 and OC43, adenovirus, influenza viruses A and B, human respiratory syncytial viruses A and B, and human rhinovirus A. The analytical sensitivity of the assay was 10–100 copies per reaction for each type of virus. There was no cross-reactivity with common bacterial or viral pathogens. A comparison with conventional viral culture and immunofluorescence was carried out using 101 respiratory specimens from 92 patients. Using viral culture, 57 specimens (56.4%) were positive without co-infection. The same viruses were identified in all 57 specimens using the multiplex PCR. Seven of the 57 specimens (12.3%) were found to be co-infected with other respiratory viruses, and 19 of 44 (43.2%) specimens which were negative by culture were positive by the multiplex PCR. The Seeplex Respiratory Virus Detection assay represents a significant improvement over the conventional methods for the detection of a broad spectrum of respiratory viruses.
has issue date	2008-12-24(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2008.11.007
bibo:pmid	19063921
has license	no-cc
sha1sum (hex)	4a8273e76da5168831b4e2a2b6887f79f312f936
schema:url	https://doi.org/10.1016/j.jviromet.2008.11.007
resource representing a document's title	Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay
has PubMed Central identifier	PMC7112863
has PubMed identifier	19063921
schema:publication	J Virol Methods
resource representing a document's body	covid:4a8273e76da5168831b4e2a2b6887f79f312f936#body_text
is schema:about of	named entity 'respiratory infections' named entity 'DUAL' named entity 'IDENTIFICATION' named entity 'USING' covid:arg/4a8273e76da5168831b4e2a2b6887f79f312f936 named entity 'DIAGNOSIS' named entity 'BASED' named entity 'TIMELY' named entity 'COMMON' named entity 'ACUTE' named entity 'RESPIRATORY INFECTIONS' named entity 'RAPID' named entity 'PCR ASSAY' named entity 'PRIMING' named entity 'OLIGONUCLEOTIDE' named entity 'DETECTION' named entity 'RESPIRATORY VIRUSES' named entity 'SYSTEM' named entity 'MULTIPLEX PCR' named entity 'PROVIDING' named entity 'VIRAL' named entity 'ACCURATE' named entity 'IMPORTANT' named entity 'CAUSES' named entity 'THERAPEUTIC INTERVENTIONS' named entity 'HUMAN DISEASE' named entity 'RAPID' named entity 'human' named entity 'diagnosis' named entity 'respiratory infections' named entity 'viruses' named entity 'multiplex PCR' named entity 'oligonucleotide' named entity 'PCR-based methods' named entity 'viruses' named entity 'virus' named entity 'reverse transcriptase' named entity 'RNA' named entity 'Reverse transcription' named entity 'viral culture' named entity 'adenoviruses' named entity 'immunofluorescence' named entity 'respiratory syncytial viruses' named entity 'respiratory tract infections' named entity 'plasmid DNA' named entity 'RSV' named entity 'respiratory symptoms' named entity 'laryngeal carcinoma' named entity 'multiplex PCR' named entity 'lower respiratory tract infections' named entity 'chest radiograph' named entity 'multiplex PCR' named entity 'nosocomial' named entity 'viruses' named entity 'primers' named entity 'Co-infection' named entity 'viral culture' named entity 'antigens' named entity 'plasmid DNA' named entity 'PCR' named entity 'viruses' named entity 'RNA viruses' named entity 'human genomic' named entity 'multiplex PCR' named entity 'viral culture' named entity 'Canada' named entity 'Virus' named entity 'vector' named entity 'MDCK' named entity 'co-infection' named entity 'RSV' named entity 'gold standard' named entity 'positive control' named entity 'microorganisms' named entity 'RSV' named entity 'co-infection' named entity 'Fermentas' named entity 'Seoul' named entity 'viruses'

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