About: Identification of effective siRNA blocking the expression of SARS viral envelope E and RDRP genes

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About: Identification of effective siRNA blocking the expression of SARS viral envelope E and RDRP genes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of effective siRNA blocking the expression of SARS viral envelope E and RDRP genes
Creator	Meng, Bo Hu, Yinghe Cao, Changxiu Lui, Yue-Woon Meng, Shi
Source	Medline; PMC
abstract	A cell-based assay was developed to screen small interference RNA (siRNA) to block the expression of two genes of the severe acute respiratory syndrome (SARS) virus. These two genes encode RNA-dependent RNA polymerase (RDRP) and envelope E protein. The RDRP plays an essential role in viral RNA replication where envelope E protein is involved in envelope formation and virus assembly. The RDRP and envelope E genes, based on published sequences, have been synthesized and cloned into mammalian expression vectors. In addition, four siRNA sites for the RDRP gene and two siRNA sites for envelope E gene were designed and tested. The siRNA or short hairpin RNA (shRNA) expression cassettes were co-transfected with the SARS viral RDRP or envelope E expression vectors into NIH 3T3 cells. The expression levels of RDRP and envelope E genes were examined by reverse transcription followed by quantitative real-time polymerase chain reaction (PCR). Two of the siRNA expression cassettes for RDRP successfully inhibited the expression of the gene, whereas both of the siRNA expression cassettes for envelope E decreased approx 90% of the envelope E gene expression. The siRNA and shRNA for one of the siRNA sites of the RDRP gene were also tested, and it was found that both inhibited exogenous RDRP expression in a dose-dependent manner. These siRNA molecules could be used to examine the function of these genes in SARS virus replication and assembly. Furthermore, these molecules could potentially be developed into therapeutic agents for the treatment of patients with SARS.
has issue date	2006-01-01(xsd:dateTime)
bibo:doi	10.1385/mb:33:2:141
bibo:pmid	16757801
has license	no-cc
sha1sum (hex)	489386f4d2ef5fd51ca4370a3957a9ca1911487c
schema:url	https://doi.org/10.1385/mb:33:2:141
resource representing a document's title	Identification of effective siRNA blocking the expression of SARS viral envelope E and RDRP genes
has PubMed Central identifier	PMC7090727
has PubMed identifier	16757801
schema:publication	Mol Biotechnol
resource representing a document's body	covid:489386f4d2ef5fd51ca4370a3957a9ca1911487c#body_text
is schema:about of	named entity 'BLOCKING' named entity 'PCR' named entity 'cloned' named entity 'NIH' named entity 'function' named entity 'genes' named entity 'RDRP' named entity 'formation' named entity 'MOLECULAR BIOTECHNOLOGY' named entity 'REVERSE TRANSCRIPTION' named entity 'EXPRESSION' named entity 'ADDITION' named entity 'SIRNA' named entity 'COULD BE' named entity 'RNA-DEPENDENT RNA POLYMERASE' named entity 'CLONED' named entity 'NIH 3T3 CELLS' named entity 'FORMATION' named entity 'SEQUENCES' named entity 'TREATMENT' named entity 'ASSEMBLY' named entity 'SMALL' named entity 'APPROX' named entity 'PLAYS' named entity 'SHORT HAIRPIN RNA' named entity 'SHRNA' named entity 'SARS' named entity '141' covid:arg/489386f4d2ef5fd51ca4370a3957a9ca1911487c named entity 'ENCODE' named entity 'SARS' named entity 'EXOGENOUS' named entity 'ENVELOPE' named entity 'INTERFERENCE RNA' named entity 'EXAMINED' named entity 'EFFECTIVE' named entity 'GENES' named entity 'SIRNA' named entity 'EXPRESSION' named entity 'MOLECULAR' named entity 'RDRP' named entity 'BIOTECHNOLOGY' named entity 'IDENTIFICATION' named entity 'PUBLISHED' named entity 'CASSETTES' named entity 'USED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'VIRAL' named entity 'HAVE' named entity 'GENE EXPRESSION' named entity 'THERAPEUTIC AGENTS' named entity 'VIRAL ENVELOPE' named entity 'INHIBITED' named entity 'TO EXAMINE' named entity 'MAMMALIAN' named entity 'FOUND' named entity 'TESTED' named entity 'SARS VIRUS' named entity 'VIRUS REPLICATION' named entity 'VIRAL RNA' named entity 'A CELL' named entity 'E PROTEIN' named entity 'PROTEIN ' named entity 'ROLE' named entity 'VIRUS' named entity 'BASED' named entity 'VIRUS ASSEMBLY' named entity 'SYNTHESIZED' named entity 'THESE' named entity 'SCREEN' named entity 'GENES' named entity 'FOLLOWED BY' named entity 'ONE OF' named entity 'ESSENTIAL' named entity 'EXPRESSION VECTORS' named entity 'RDRP' named entity 'RNA REPLICATION'

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