About: Quantitative and qualitative flow cytometric analysis of nanosized cell-derived membrane vesicles

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Quantitative and qualitative flow cytometric analysis of nanosized cell-derived membrane vesicles
Creator	Bartelink, Willem Aalberts, Marian Mastrobattista, Enrico Stoorvogel, Willem Bosch, Jan Arkesteijn, J Mertens, Hendrik Nolte-'t Hoen, Esther Van Der Vlist, Els Van Gaal, Ethlinn Wauben, Marca
source	Elsevier; Medline; PMC
abstract	Nanosized cell-derived membrane vesicles are increasingly recognized as therapeutic vehicles and high-potential biomarkers for several diseases. Currently available methods allow bulk analysis of vesicles but are not suited for accurate quantification and fail to reveal phenotypic heterogeneity in membrane vesicle populations. For such analyses, single vesicle-based, multiparameter, high-throughput methods are needed. We developed a fluorescence-based, high-resolution flow cytometric method for quantitative and qualitative analysis of nanosized membrane vesicles. Proof of principle was obtained by single-particle analysis of virions and liposomes. Further validation was obtained by quantification of cell-derived nanosized membrane vesicles from cell cultures and body fluids. An important aspect was that the technology was extended to detect specific proteins on individual vesicles. This allowed identification of exosome subsets and phenotyping of individual exosomes produced by dendritic cells (DCs) undergoing different modes of activation. The described technology allows quantitative, multiparameter, and high-throughput analysis of a wide variety of nanosized particles and has broad applications. FROM THE CLINICAL EDITOR: The authors developed a fluorescence-based, high-resolution flow cytometric method for quantitative and qualitative analysis of nanosized cell-derived membrane vesicles that are increasingly recognized both as therapeutic vehicles and high-potential biomarkers for several diseases. A high throughput, easily available, and sensitive detection method such as the one discussed here is a critically important prerequisite for further refinements of this technology.
has issue date	2011-10-21(xsd:dateTime)
bibo:doi	10.1016/j.nano.2011.09.006
bibo:pmid	22024193
has license	no-cc
sha1sum (hex)	47fa139b0aea8bbe6fcdec7eeeb082384b7f9fd0
schema:url	https://doi.org/10.1016/j.nano.2011.09.006
resource representing a document's title	Quantitative and qualitative flow cytometric analysis of nanosized cell-derived membrane vesicles
has PubMed Central identifier	PMC7106164
has PubMed identifier	22024193
schema:publication	Nanomedicine
resource representing a document's body	covid:47fa139b0aea8bbe6fcdec7eeeb082384b7f9fd0#body_text
is schema:about of	named entity 'reveal' named entity 'broad' named entity 'dendritic cells' named entity 'therapeutic' named entity 'validation' named entity 'single' named entity 'MEMBRANE VESICLE' named entity 'VARIETY' named entity 'PHENOTYPIC HETEROGENEITY' named entity 'ACCURATE' named entity 'EXTENDED' named entity 'FLOW CYTOMETRIC' named entity 'METHODS' named entity 'TO DETECT' named entity 'SINGLE' named entity 'VIRIONS' named entity 'TECHNOLOGY' named entity 'DESCRIBED' named entity 'ACTIVATION' named entity 'PHENOTYPING' named entity 'QUALITATIVE ANALYSIS' named entity 'DCs' named entity 'membrane' named entity 'vesicles' named entity 'modes' named entity 'membrane' named entity 'membrane' named entity 'analyses' named entity 'high-throughput methods' named entity 'high-throughput' named entity 'vesicles' named entity 'membrane vesicles' named entity 'flow cytometric' named entity 'membrane vesicles' named entity 'membrane vesicles' named entity 'membrane vesicles' named entity 'hepatitis' named entity 'light scattering' named entity 'exosomes' named entity 'fluorescence' named entity 'C57BL/6' named entity 'virions' named entity 'co-cultured' named entity 'fluorescence' named entity 'body fluid' named entity 'vesicles' named entity 'calcein' named entity 'flow cytometry' named entity 'biomarker' named entity 'NTA' named entity 'biological fluids' named entity 'flow cytometry' named entity 'PBS' named entity 'assay' named entity 'peptide' named entity 'Light scattering' named entity 'flow cytometric' named entity 'quality control' named entity 'vesicle' named entity 'Prof.' named entity 'fluorescence' named entity 'prostate' named entity 'clinical trials' named entity 'Microscopic analysis' named entity 'exosomes' named entity 'liposomes' named entity 'BSA' named entity 'vesicles' named entity 'membrane vesicles' named entity 'flow cytometer' named entity 'fluorescence'

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