About: Early and late HHV-6 gene transcripts in multiple sclerosis lesions and normal appearing white matter

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Early and late HHV-6 gene transcripts in multiple sclerosis lesions and normal appearing white matter
Creator	Kennedy, Peter Opsahl, Margaret
Source	Medline; PMC
abstract	Multiple sclerosis is an inflammatory demyelinating disease of the CNS, the aetiology of which is believed to have both genetic and environmental components. We have investigated one of the candidate viruses for the environmental component of multiple sclerosis, the neurotropic human herpesvirus 6 (HHV-6). Utilizing fluorescent in situ hybridization (FISH) techniques, we have examined human post-mortem tissues for the presence of immediate early and late viral gene expression in multiple sclerosis patient normal appearing white matter (NAWM), lesional tissue and normal control brain samples. HHV-6 gene transcription was detected in all tissue samples and was restricted to oligodendrocytes, as determined by double mRNA FISH analysis. Quantitative analysis of viral mRNA expression indicated that both NAWM and lesional multiple sclerosis samples exhibited significantly higher levels of HHV-6 expression compared with the normal control samples. Lesional samples exhibited the highest levels of viral gene expression, with NAWM exhibiting an intermediate level between lesional and control tissues. Immunofluorescence against early and late HHV-6 proteins verified active translation of HHV-6 viral mRNA in oligodendrocytes. Southern blot analysis of nested polymerase chain reactions using extracted genomic DNA and cDNA confirmed the presence of the HHV-6 genome in all individuals, with the active expression profile mirroring the FISH results. The frequent high level of HHV-6 infection in multiple sclerosis samples suggests a possible role in pathogenesis.
has issue date	2005-01-19(xsd:dateTime)
bibo:doi	10.1093/brain/awh390
bibo:pmid	15659422
has license	no-cc
sha1sum (hex)	46d84eb89cf37fb5ed31a4607c5b2c3da699953f
schema:url	https://doi.org/10.1093/brain/awh390
resource representing a document's title	Early and late HHV-6 gene transcripts in multiple sclerosis lesions and normal appearing white matter
has PubMed Central identifier	PMC7109784
has PubMed identifier	15659422
schema:publication	Brain
resource representing a document's body	covid:46d84eb89cf37fb5ed31a4607c5b2c3da699953f#body_text
is schema:about of	named entity 'white matter' named entity 'multiple sclerosis' named entity 'cDNA' named entity 'hapten' named entity 'single cells' named entity 'lesion' named entity 'HHV-6' named entity 'nested PCR' named entity 'oligodendrocytes' named entity 'RNA' named entity 'viral infection' named entity 'multiple sclerosis' named entity 'viral infection' named entity 'HHV-6' named entity 'viral DNA' named entity 'PCR' named entity 'coupled receptor' named entity 'NAWM' named entity 'endogenous retroviruses' named entity 'AbCam' named entity 'subspecies' named entity 'multiple sclerosis' named entity 'multiple sclerosis' named entity 'HHV-6B' named entity 'virus' named entity 'infection' named entity 'rat' named entity 'genomic DNA' named entity 'rhodamine' named entity 'post-mortem' named entity 'PCR' named entity 'nested PCR' named entity 'HHV-6' named entity 'mRNA' named entity 'oligodendrocytes' named entity 'mRNA' named entity 'HHV-6' named entity 'gene expression' named entity 'neurodegeneration' named entity 'oligo' named entity 'viral infection' named entity 'lesion' named entity 'mRNA' named entity 'rhodamine' named entity 'serum' named entity 'multiple sclerosis' named entity 'HHV-6' named entity 'pathogenesis' named entity 'rodent' named entity 'oligodendrocytes' named entity 'fluorescein' named entity 'rhodamine' named entity 'housekeeping gene' named entity 'gene expression' named entity 'phosphate' named entity '2-phenylindole' named entity 'seroprevalence' named entity 'HHV-6' named entity 'viral genome' named entity 'primers' named entity 'multiple sclerosis' named entity 'FISH' named entity 'multiple sclerosis' named entity 'high levels' named entity 'constitutively active' named entity 'mRNA' named entity 'herpesvirus' named entity 'RNA' named entity 'multiple sclerosis' named entity 'subspecies' named entity 'HHV-6' named entity 'HHV-6' named entity 'gene expression' named entity 'DAPI' named entity 'measles, mumps' named entity 'IgG' named entity 'post-mortem interval' named entity 'white matter' named entity 'HHV-6' named entity 'oligodendrocytes'

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