About: CD13/Aminopeptidase N overexpression by basic fibroblast growth factor mediates enhanced invasiveness of 1F6 human melanoma cells

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About: CD13/Aminopeptidase N overexpression by basic fibroblast growth factor mediates enhanced invasiveness of 1F6 human melanoma cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	CD13/Aminopeptidase N overexpression by basic fibroblast growth factor mediates enhanced invasiveness of 1F6 human melanoma cells
Creator	Boven, E Fontijn, D Yuana, Y Broxterman, H Duyndam, Mca Pinedo, H Shapiro, L Van Berkel, Mpa
source	Medline; PMC
abstract	CD13/Aminopeptidase N (CD13) is known to play an important role in tumour cell invasion. We examined whether basic fibroblast growth factor (bFGF) is involved in the regulation of CD13 expression in human melanoma cells. 1F6 human melanoma cells were stably transfected with constructs encoding either the 18 kDa (18kD) or all (ALL) bFGF isoform proteins. We observed highly increased CD13 mRNA and protein expression in the 1F6 clones regardless of the overexpression of either the 18kD or all isoform proteins. Neutral aminopeptidase activity was increased five-fold and could be inhibited by bestatin and the CD13-neutralising antibody WM15. The enhanced invasion through Matrigel, but not migration in a wound assay, was efficiently abrogated by both bestatin and WM15. Upregulation of CD13 expression was the result of increased epithelial and myeloid promoter activity up to 4.5-fold in 1F6-18kD and 1F6-ALL clones. Interestingly, in a panel of human melanoma cell lines, a significant correlation (r(2)=0.883, P<0.05) between bFGF and CD13 mRNA and protein expression was detected. High bFGF and CD13 expression were clearly related with an aggressive phenotype. Taken together, our data indicate that high bFGF expression upregulates CD13 expression in human melanoma cells by activating both the myeloid and the epithelial CD13 promoter. In addition, we show that high bFGF and CD13 expression results in enhanced invasive capacity and metastatic behaviour of human melanoma cells.
has issue date	2006-05-09(xsd:dateTime)
bibo:doi	10.1038/sj.bjc.6603157
bibo:pmid	16685268
has license	no-cc
sha1sum (hex)	4598e96f24115b1fd9bc0247cdd88e52beb5268f
schema:url	https://doi.org/10.1038/sj.bjc.6603157
resource representing a document's title	CD13/Aminopeptidase N overexpression by basic fibroblast growth factor mediates enhanced invasiveness of 1F6 human melanoma cells
has PubMed Central identifier	PMC2361307
has PubMed identifier	16685268
schema:publication	Br J Cancer
resource representing a document's body	covid:4598e96f24115b1fd9bc0247cdd88e52beb5268f#body_text
is schema:about of	named entity 'epithelial' named entity 'protein' named entity 'activating' named entity 'bFGF' named entity 'fibroblast' named entity 'constructs' named entity 'Matrigel' named entity 'expression' named entity 'abrogated' named entity 'enhanced' named entity 'ALL' named entity 'high' named entity 'migration' named entity 'result' named entity 'expression' named entity 'CD13' named entity 'CD13' named entity 'human' covid:arg/4598e96f24115b1fd9bc0247cdd88e52beb5268f named entity 'melanoma' named entity 'cell lines' named entity 'cell' named entity 'transfected' named entity 'melanoma' named entity 'human' named entity 'conjugated' named entity 'bFGF' named entity 'bestatin' named entity 'mRNA expression' named entity 'ATCC' named entity 'epithelial' named entity 'Austria' named entity 'bFGF' named entity 'p38 MAPK' named entity 'Taq DNA polymerase' named entity 'distal' named entity 'mRNA' named entity 'activity' named entity 'CD13' named entity 'growth factor' named entity 'cells' named entity 'CD13' named entity 'enhanced' named entity 'promoter' named entity 'expression' named entity 'Aminopeptidase N' named entity 'aminopeptidase' named entity 'bFGF' named entity 'protein expression' named entity 'bFGF' named entity 'assay' named entity 'melanoma' named entity 'CD13' named entity 'bestatin' named entity 'phenotype' named entity 'myeloid' named entity 'CD13' named entity 'kDa' named entity 'CD13' named entity 'melanoma cells' named entity 'clones' named entity 'melanoma cells' named entity 'basic fibroblast growth factor' named entity 'bestatin' named entity 'aminopeptidase' named entity 'CD13' named entity 'nucleus' named entity 'renal-cell carcinoma' named entity 'cell lines' named entity 'antitumour' named entity 'transcription factors' named entity 'bFGF' named entity 'IgG1' named entity 'epithelial cells'

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