About: Synthesis of a bacteriophage MB78 late protein by novel ribosomal frameshifting()

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About: Synthesis of a bacteriophage MB78 late protein by novel ribosomal frameshifting() Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Synthesis of a bacteriophage MB78 late protein by novel ribosomal frameshifting()
Creator	Chakravorty, Maharani Kolla, Venkatadri Litwack, Gerald Mukherjee, Annapurna Pandey, Bindu Srinivasula, Srinivasa
Source	Elsevier; Medline; PMC
abstract	MB78 is a virulent phage of Salmonella typhimurium that possesses a number of interesting features, making it a suitable organism to study the regulation of gene expression. A detailed physical map of this phage genome has been constructed and is being extensively studied at the molecular level. Here, we demonstrate the expression of two late proteins of bacteriophage MB78 derived from the same gene as a result of possible ribosomal frameshifting. In vitro transcription-translation yields a major protein that migrates as 28 kDa, whereas in vivo expression using pET expression vectors yields two equally expressed proteins of molecular sizes 28 and 26 kDa. A putative slippery sequence TTTAAAG and a pseudoknot structure, two essential cis elements required for the classical ribosomal frameshifting, are identified in the reading frame. Mutations created at the slippery sequence resulted in a single 28 kDa protein and completely abolished the expression of 26 kDa protein. Thus, we have produced the first evidence that ribosomal frameshifting occurs in bacteriophage MB78 of Salmonella typhimurium.
has issue date	2000-08-22(xsd:dateTime)
bibo:doi	10.1016/s0378-1119(00)00264-x
bibo:pmid	10974552
has license	no-cc
sha1sum (hex)	45713f9e6085b078e4dc902790cf3e5cdca43151
schema:url	https://doi.org/10.1016/s0378-1119%2800%2900264-x
resource representing a document's title	Synthesis of a bacteriophage MB78 late protein by novel ribosomal frameshifting()
has PubMed Central identifier	PMC7173172
has PubMed identifier	10974552
schema:publication	Gene
resource representing a document's body	covid:45713f9e6085b078e4dc902790cf3e5cdca43151#body_text
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