About: Translational diffusion of class II major histocompatibility complex molecules is constrained by their cytoplasmic domains

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About: Translational diffusion of class II major histocompatibility complex molecules is constrained by their cytoplasmic domains Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Translational diffusion of class II major histocompatibility complex molecules is constrained by their cytoplasmic domains
Creator	Freed, John Wade, William
Source	PMC
abstract	Site-directed mutagenesis in vitro was used to introduce stop codons in the genomic DNA of the alpha and beta chains of the murine class II major histocompatibility complex antigen, I-Ak. Mutated DNA was transfected into B lymphoma cells that were then selected by neomycin resistance and for their ability to express I-Ak molecules on their plasma membrane. The translational diffusion coefficient (Dlat) of I-Ak molecules composed of a wild-type beta chain paired with an alpha chain missing either 6 or 12 amino acids from the cytoplasmic domain is on the average threefold higher than the Dlat of wild-type I-Ak molecules as measured by fluorescence photobleaching and recovery. The removal of 12 amino acids from the cytoplasmic domain of the beta chain did not change the Dlat value from that of wild-type I-Ak if the truncated beta chain was paired with a wild-type alpha chain. Removing all amino acids of the cytoplasmic domains of both the alpha and beta chains resulted in a 10-fold increase in the Dlat, the highest value for any of the truncated I-Ak molecules tested. These data indicate that the carboxy- terminal six amino acids of the cytoplasmic domain of the alpha chain and the six plasma membrane-proximal amino acids of the beta chain are important in constraining the translational diffusion of I-Ak molecules in the plasma membrane.
has issue date	1989-12-01(xsd:dateTime)
bibo:pmid	2557353
has license	cc-by-nc-sa
sha1sum (hex)	44ca0c52f79a893a551da92dd78e599fbc74b060
resource representing a document's title	Translational diffusion of class II major histocompatibility complex molecules is constrained by their cytoplasmic domains
has PubMed Central identifier	PMC2115898
has PubMed identifier	2557353
schema:publication	J Cell Biol
resource representing a document's body	covid:44ca0c52f79a893a551da92dd78e599fbc74b060#body_text
is schema:about of	named entity 'DIFFUSION COEFFICIENT' named entity 'TRANSLATIONAL' named entity 'DIFFUSION' named entity 'AMINO ACIDS' named entity 'NEOMYCIN' named entity 'TYPE I' named entity 'PHOTOBLEACHING' named entity 'CHAINS' named entity 'PROXIMAL' named entity 'CARBOXY' named entity 'SELECTED' named entity 'CHAIN' named entity 'RESISTANCE' named entity 'DID' named entity 'I-A' named entity 'resistance' named entity 'chain' named entity 'Site-directed mutagenesis' named entity 'domain' named entity 'neomycin' named entity 'THEIR' covid:arg/44ca0c52f79a893a551da92dd78e599fbc74b060 named entity 'PAIRED' named entity 'RECOVERY' named entity 'IN VITRO' named entity 'TRANSLATIONAL' named entity 'USED' named entity 'FOLD INCREASE' named entity 'THEIR' named entity 'TRUNCATED' named entity 'PLASMA MEMBRANE' named entity 'TO EXPRESS' named entity 'EITHER' named entity 'MAJOR HISTOCOMPATIBILITY COMPLEX' named entity 'DIFFUSION' named entity 'MOLECULES' named entity 'CLASS II MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGEN' named entity 'HIGHER' named entity 'MISSING' named entity 'MOLECULES' named entity 'REMOVING' named entity 'IMPORTANT' named entity 'TESTED' named entity 'CYTOPLASMIC DOMAIN' named entity 'SITE-DIRECTED MUTAGENESIS' named entity 'MUTATED' named entity 'TERMINAL' named entity 'REMOVAL' named entity 'CHANGE' named entity 'VALUE' named entity 'INTRODUCE' named entity 'AVERAGE' named entity 'CLASS II' named entity 'CYTOPLASMIC' named entity 'CONSTRAINED' named entity 'MEASURED' named entity 'STOP CODONS' named entity 'HIGHEST' named entity 'COMPOSED OF' named entity 'TYPE C' named entity 'FLUORESCENCE' named entity 'MURINE' named entity 'DNA' named entity 'WILD-TYPE' named entity 'CYTOPLASMIC' named entity 'GENOMIC DNA' named entity 'DATA' named entity 'LYMPHOMA CELLS' named entity 'THESE' named entity 'The' named entity 'DNA' named entity 'measured' named entity 'missing' named entity 'major histocompatibility complex' named entity 'molecules' named entity 'plasma membrane' named entity 'These' named entity 'cells' named entity 'recovery' named entity 'average' named entity 'chain' named entity 'I-A'

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