About: Leishmania infantum-specific IFN-γ production in stimulated blood from cats living in areas where canine leishmaniosis is endemic

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About: Leishmania infantum-specific IFN-γ production in stimulated blood from cats living in areas where canine leishmaniosis is endemic Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Leishmania infantum-specific IFN-γ production in stimulated blood from cats living in areas where canine leishmaniosis is endemic
Creator	Solano-Gallego, Laia Martínez-Orellana, Pamela Bruno, Federica Castelli, Germano Ippolito, Dorotea Masucci, Marisa Prandi, David Priolo, Vito Pennisi, Maria
Source	Medline; PMC
abstract	BACKGROUND: Feline leishmaniosis caused by Leishmania infantum is considered a rare disease in endemic areas, whereas subclinical infections are common. Immune response plays a key role in driving the course of L. infantum infection in other host species; however, the feline cell-mediated immune response to L. infantum infection has not yet been investigated. The aim of this study was to determine the cell-mediated immune response specific to L. infantum by means of interferon (IFN)-γ release in whole blood assay from cats living in endemic areas (66 in Sicily and 113 in Catalonia) and to compare with antibody levels to L. infantum [enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT)], blood parasite load and retroviral infections. RESULTS: Most cats (n = 140) were L. infantum antibody negative and only 22% (n = 39) were positive. Only 9 and 2% of tested cats had a feline immunodeficency virus (FIV) infection or a feline leukemia virus (FeLV) infection, respectively. Thirty-two cats out of 179 (18%) produced IFN-γ after stimulation with L. infantum soluble antigen (LSA) while the majority of cats (93%) produced IFN-γ after stimulation with concanavalin A (ConA). Six LSA-IFN-γ-producer cats were seropositive (three to ELISA and five to IFAT) but they were polymerase chain reaction (PCR) negative, while only one cat was antibody- and PCR-positive. Significant positive correlations were found between IFN-γ concentrations after stimulation with LSA and ConA, and between serology and PCR testing. No association was found between FIV status and LSA or ConA-IFN-γ production. Combining PCR, serology and specific IFN-γ concentration results, we found that 36% of cats studied were exposed to L. infantum. CONCLUSIONS: As expected, cats from endemic areas produce IFN-γ after ex vivo blood stimulation with LSA and therefore are able to activate a cell-mediated adaptive immune response against the parasite that is variably associated with antibody or blood PCR positivity. The association of this assay to serological and molecular tests provides a better estimate of cat exposure to L. infantum.
has issue date	2019-03-26(xsd:dateTime)
bibo:doi	10.1186/s13071-019-3386-y
bibo:pmid	30909952
has license	cc-by
sha1sum (hex)	43fd9e9fa6ecb2ec700c15288e7e3c7ba38f26a2
schema:url	https://doi.org/10.1186/s13071-019-3386-y
resource representing a document's title	Leishmania infantum-specific IFN-γ production in stimulated blood from cats living in areas where canine leishmaniosis is endemic
has PubMed Central identifier	PMC6434818
has PubMed identifier	30909952
schema:publication	Parasit Vectors
resource representing a document's body	covid:43fd9e9fa6ecb2ec700c15288e7e3c7ba38f26a2#body_text
is schema:about of	named entity 'retroviral infections' named entity 'assay' named entity 'Immune response' named entity 'Leishmania infantum' named entity 'key' named entity 'immunofluorescence' named entity 'canine' named entity 'STIMULATED' named entity 'PRODUCTION' named entity 'COMMON' named entity 'LEVELS' named entity 'CELL-MEDIATED IMMUNE RESPONSE' named entity 'BACKGROUND' named entity 'AREAS' named entity 'HOST SPECIES' named entity 'KEY' named entity 'IMMUNE RESPONSE' named entity 'PARASITE LOAD' named entity 'DETERMINE' named entity 'COURSE' named entity 'RELEASE' named entity 'SUBCLINICAL' named entity 'BLOOD ASSAY' named entity 'INTERFERON ' named entity 'CAUSED BY' named entity 'CATS' named entity 'ROLE' named entity 'CONSIDERED' named entity 'LEISHMANIOSIS' named entity 'WHOLE BLOOD' named entity 'INFECTION' named entity 'THE CELL' named entity 'RETROVIRAL INFECTIONS' named entity 'L. INFANTUM' named entity 'RESPONSE TO' named entity 'LIVING' named entity 'CATS' named entity 'LEISHMANIOSIS' named entity 'WHERE' named entity 'BLOOD' named entity 'CANINE' named entity 'AREAS' named entity 'SPECIFIC' named entity 'SPECIFIC' named entity 'RARE DISEASE' named entity 'IMMUNOSORBENT' named entity 'STUDY' named entity 'ANTIBODY' named entity 'BLOOD PARASITE' named entity 'ANTIBODY TEST' named entity 'MEANS' named entity 'FELINE' named entity 'IMMUNOFLUORESCENCE' named entity 'LEISHMANIA INFANTUM' named entity 'INVESTIGATED' named entity 'ASSAY' named entity 'ENDEMIC' named entity 'ENDEMIC' named entity 'DRIVING' named entity 'IFN' named entity 'LEISHMANIA INFANTUM' named entity 'LIVING' named entity 'TO COMPARE' named entity 'INFECTIONS' named entity 'PLAYS' named entity 'SICILY' named entity 'AIM' covid:arg/43fd9e9fa6ecb2ec700c15288e7e3c7ba38f26a2 named entity 'driving' named entity 'specific' named entity 'cell-mediated immune response' named entity 'interferon' named entity 'antibody'

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