About: Diagnostic et surveillance épidémiologique des infections grippales et à virus respiratoire syncytial : intérêt de la PCR multiplex

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About: Diagnostic et surveillance épidémiologique des infections grippales et à virus respiratoire syncytial : intérêt de la PCR multiplex Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Diagnostic et surveillance épidémiologique des infections grippales et à virus respiratoire syncytial : intérêt de la PCR multiplex
Creator	Agius, G Beby-Defaux, A Giraudeau, G Godet, C Paccalin, M Plouzeau, C
Source	Elsevier; PMC
abstract	OBJECTIVE: Respiratory infections require a rapid etiological diagnosis for efficient management of cases. We evaluated multiplex PCR used for the diagnosis and the epidemiological surveillance of influenza and respiratory syncytial virus (RSV) infections. PATIENTS AND METHODS: Our study included 278 patients (mean age: 37.2 ± 22.9 years) with flu or flu-like syndromes, consulting physicians affiliated with the GROG Poitou-Charentes or hospitalized in the Poitiers teaching hospital. A multiplex PCR detecting A(H3), A(H1) and B influenza viruses, and RSV A and B, was performed with both a direct examination by immunofluorescence and cell-culture. RESULTS: We diagnosed a viral infection in 139 (50.0%) patients: 99 cases of influenza A(H3), 2 cases of influenza A(H1), 28 cases of influenza B and 11 cases of RSV infections. The diagnosis yield in GROG patients (52.3%) was significantly higher than that observed in hospitalized patients (34.5%) (P = 0.04). All techniques were correlated in 61% of cases. The multiplex PCR yielded 22.3% more positive samples compared to the conventional techniques. All positive samples by conventional techniques were also positive by multiplex PCR. We observed a perfect correlation between viral types and subtypes determined by PCR and cell-culture. CONCLUSION: Multiplex PCR is a sensitive technique allowing an efficient and rapid diagnosis of respiratory infections due to influenza and RSV.
has issue date	2007-06-08(xsd:dateTime)
bibo:doi	10.1016/j.medmal.2007.02.010
bibo:pmid	17560747
has license	no-cc
sha1sum (hex)	43b031fedcb356e63a1902751bb2f4c51804ece0
schema:url	https://doi.org/10.1016/j.medmal.2007.02.010
resource representing a document's title	Diagnostic et surveillance épidémiologique des infections grippales et à virus respiratoire syncytial : intérêt de la PCR multiplex
has PubMed Central identifier	PMC7119120
has PubMed identifier	17560747
schema:publication	Med Mal Infect
resource representing a document's body	covid:43b031fedcb356e63a1902751bb2f4c51804ece0#body_text
is schema:about of	named entity 'SURVEILLANCE' named entity 'INFLUENZA' named entity 'AAT' named entity 'MULTIPLEX' named entity 'PATIENTS' named entity 'DIAGNOSTIC' named entity 'POUR' named entity 'PCR' named entity 'DIAGNOSTIC' named entity 'EFFICACE' covid:arg/43b031fedcb356e63a1902751bb2f4c51804ece0 named entity 'INFECTIONS' named entity 'DES' named entity 'PCR' named entity 'MULTIPLEX PCR' named entity 'INT' named entity 'INTEREST' named entity 'VIRUS RESPIRATOIRE SYNCYTIAL' named entity 'RESPIRATORY SYNCYTIAL VIRUS INFECTIONS' named entity 'VIRUS RESPIRATOIRE SYNCYTIAL' named entity 'RAPIDEMENT' named entity 'EPIDEMIOLOGICAL' named entity 'DES' named entity 'DIAGNOSIS' named entity 'MULTIPLEX' named entity 'INFECTIONS' named entity 'UNE' named entity 'PRISE EN CHARGE' named entity 'epidemiological surveillance' named entity 'syncytial' named entity 'influenza' named entity 'permet' named entity 'direct examination' named entity 'agarose' named entity 'Biorad' named entity 'permet' named entity 'PCRm' named entity 'centrifugation' named entity 'région' named entity 'immunofluorescence assay' named entity 'PCRm' named entity '1, 2' named entity 'PCRm' named entity 'PCR' named entity 'PCRm' named entity 'influenza' named entity '100 %' named entity 'PCRm' named entity 'immunofluorescence' named entity 'Center for Disease Control' named entity 'PCR' named entity 'permet' named entity 'PCR' named entity 'Chemicon' named entity 'mis' named entity 'Influenza' named entity 'PCR' named entity 'Poitou-Charentes' named entity 'PCRm' named entity 'PCR' named entity 'H3N2' named entity 'tampon' named entity 'la présentation' named entity 'PCR' named entity 'influenza' named entity 'PCRm' named entity 'Sichuan' named entity 'permis' named entity 'PCR' named entity 'PCRm' named entity 'PCR' named entity 'MRC-5' named entity '1, 2' named entity 'mise en place' named entity 'PCR' named entity 'le temps'

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