About: Protection from lethal coronavirus infection by affinity-purified spike glycoprotein of murine hepatitis virus, strain A59

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About: Protection from lethal coronavirus infection by affinity-purified spike glycoprotein of murine hepatitis virus, strain A59 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Protection from lethal coronavirus infection by affinity-purified spike glycoprotein of murine hepatitis virus, strain A59
Creator	Danieland, Claude Talbot', Pierre
topic	covid:43ac831b9f0a365a772c810673eae7dfdff6b7ba#this
source	Elsevier; Medline; PMC
abstract	Abstract Murine hepatitis viruses provide excellent animal models for the study of virus-induced diseases of the central nervous system and gastrointestinal tract. Several studies have indirectly provided evidence that the spike glycoprotein (S) of these coronaviruses bears determinants for pathogenesis and the induction of protective immunity. In order to directly evaluate the immunogenicity of this protein, it was purified by affinity chromatography with an in vitro neutralizing and in vivo protective monoclonal antibody which immunoprecipitated the 180-kDa spike glycoprotein of the neurotropic A59 strain of murine hepatitis virus (MHV-A59). Mice immunized twice with approximately 1 μg of purified S in Freund's adjuvant developed high titers of neutralizing and fusion inhibiting antibodies, even though the protein was at least partially denaturated after elution from the affinity column. Moreover, these mice were protected from lethal encephalitis when challenged intracerebrally with 10 LD50 of MHV-A59. This study provides a direct demonstration of the importance of the coronavirus spike glycoprotein in the induction of a protective immune response.
has issue date	1990-01-31(xsd:dateTime)
bibo:doi	10.1016/0042-6822(90)90057-x
bibo:pmid	2152996
has license	els-covid
sha1sum (hex)	43ac831b9f0a365a772c810673eae7dfdff6b7ba
schema:url	https://doi.org/10.1016/0042-6822%2890%2990057-x
resource representing a document's title	Protection from lethal coronavirus infection by affinity-purified spike glycoprotein of murine hepatitis virus, strain A59
has PubMed Central identifier	PMC7131235
has PubMed identifier	2152996
schema:publication	Virology
resource representing a document's body	covid:43ac831b9f0a365a772c810673eae7dfdff6b7ba#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/43ac831b9f0a365a772c810673eae7dfdff6b7ba
is schema:about of	named entity 'CORONAVIRUS INFECTION' named entity 'AFFINITY' named entity 'SPIKE' named entity 'DIRECT' named entity 'THESE' named entity 'PROTEIN' named entity 'IMMUNIZED' named entity 'BEARS' named entity 'CENTRAL NERVOUS SYSTEM' named entity 'DIRECTLY' named entity 'PATHOGENESIS' named entity 'IMMUNOGENICITY' named entity 'PURIFIED' covid:arg/43ac831b9f0a365a772c810673eae7dfdff6b7ba named entity '180' named entity 'PURIFIED' named entity 'LETHAL' named entity 'MURINE HEPATITIS VIRUS' named entity 'A59' named entity 'IMMUNE RESPONSE' named entity 'ADJUVANT' named entity 'MURINE HEPATITIS VIRUS' named entity 'APPROXIMATELY' named entity 'VIRUS' named entity 'ORDER' named entity 'HEPATITIS VIRUSES' named entity 'STRAIN' named entity 'PROTECTION' named entity 'FUSION' named entity 'S IN' named entity 'PARTIALLY' named entity 'DEMONSTRATION' named entity 'INHIBITING ANTIBODIES' named entity 'MURINE HEPATITIS' named entity 'IN VIVO' named entity 'CORONAVIRUSES' named entity 'ANIMAL MODELS ' named entity 'DISEASES' named entity 'AFFINITY COLUMN' named entity 'PROTECTIVE IMMUNITY' named entity 'EVALUATE' named entity 'EVIDENCE' named entity 'STRAIN' named entity 'KDA' named entity 'INDUCED' named entity 'LETHAL' named entity 'AFFINITY CHROMATOGRAPHY' named entity 'IN VITRO' named entity 'INDUCTION' named entity 'NEUROTROPIC' named entity 'CORONAVIRUS' named entity 'HIGH' named entity 'GASTROINTESTINAL TRACT' named entity 'MONOCLONAL ANTIBODY' named entity 'ELUTION' named entity 'INC' named entity '27S' named entity 'A59' named entity 'EXCELLENT' named entity '28S' named entity 'ENCEPHALITIS' named entity 'DETERMINANTS' named entity 'STUDIES' named entity 'MICE' named entity 'STUDY' named entity 'PROTEIN ' named entity 'HAVE' named entity 'PROVIDE' named entity 'TWICE' named entity 'Freund's adjuvant' named entity 'central nervous' named entity 'inocula' named entity 'contaminant' named entity 'affinity column' named entity 'biological functions' named entity 'pathogenesis' named entity 'aminopterin' named entity 'pathogenicity'

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