About: Investigation of the Role of the Spike Protein in Reversing the Virulence of the Highly Virulent Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strains and Its Attenuated Counterpart

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About: Investigation of the Role of the Spike Protein in Reversing the Virulence of the Highly Virulent Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strains and Its Attenuated Counterpart Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Investigation of the Role of the Spike Protein in Reversing the Virulence of the Highly Virulent Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strains and Its Attenuated Counterpart
Creator	Chang, Hui-Wen Kao, Chi-Fei
source	PMC
abstract	Porcine epidemic diarrhea virus (PEDV) has continuously caused severe economic losses to the global swine industries; however, no successful vaccine against PEDV has been developed. In this study, we generated four autologous recombinant viruses, including the highly virulent iPEDVPT-P5, attenuated iPEDVPT-P96, and two chimeric viruses (iPEDVPT-P5-96S and iPEDVPT-P96-5S) with the reciprocally exchanged spike (S) gene, to study the role of the S gene in PEDV pathogenesis. A deeper understanding of PEDV attenuation will aid in the rational design of a live attenuated vaccine (LAV) using reverse genetics system. Our results showed that replacing the S gene from the highly virulent iPEDVPT-P5 led to complete restoration of virulence of the attenuated iPEDVPT-P96, with nearly identical viral shedding, diarrhea pattern, and mortality rate as the parental iPEDVPT-P5. In contrast, substitution of the S gene with that from the attenuated iPEDVPT-P96 resulted in partial attenuation of iPEDVPT-P5, exhibiting similar viral shedding and diarrhea patterns as the parental iPEDVPT-P96 with slightly severe histological lesions and higher mortality rate. Collectively, our data confirmed that the attenuation of the PEDVPT-P96 virus is primarily attributed to mutations in the S gene. However, mutation in S gene alone could not fully attenuate the virulence of iPEDVPT-P5. Gene (s) other than S gene might also play a role in determining virulence.
has issue date	2019-12-30(xsd:dateTime)
bibo:doi	10.3390/v12010041
bibo:pmid	31905842
has license	cc-by
sha1sum (hex)	4393d1b0e0262247c9c6ace6ebda9dcd5395d6fa
schema:url	https://doi.org/10.3390/v12010041
resource representing a document's title	Investigation of the Role of the Spike Protein in Reversing the Virulence of the Highly Virulent Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strains and Its Attenuated Counterpart
has PubMed Central identifier	PMC7019868
has PubMed identifier	31905842
schema:publication	Viruses
resource representing a document's body	covid:4393d1b0e0262247c9c6ace6ebda9dcd5395d6fa#body_text
is schema:about of	named entity 'ITS' named entity 'vaccine' named entity 'attributed' named entity 'restoration' named entity 'gene' named entity 'viral shedding' named entity 'attenuation' named entity 'DIARRHEA' named entity 'DESIGN' named entity 'SUCCESSFUL' named entity 'CONFIRMED' named entity 'ROLE' named entity 'MUTATION' named entity '28S' named entity 'SWINE' named entity 'SEVERE' named entity 'INVESTIGATION' named entity 'HIGHLY' named entity 'USING' named entity 'ATTENUATED' named entity 'IDENTICAL' named entity 'EXCHANGED' named entity 'SIMILAR' named entity 'OUR' named entity 'GLOBAL' named entity 'CAUSED' named entity 'RESTORATION' named entity 'ATTENUATE' named entity 'COMPLETE' named entity 'LESIONS' named entity 'ROLE' named entity 'SPIKE PROTEIN' named entity 'VIRUSES' named entity 'UNDERSTANDING' named entity 'MORTALITY RATE' named entity 'TAIWAN' named entity 'PROTEIN ' named entity 'VIRULENCE' named entity 'VIRULENT' named entity 'SUBSTITUTION' named entity 'AID' named entity 'VIRULENCE' named entity 'PATTERNS' named entity 'CONTRAST' named entity 'PLAY' named entity 'RECOMBINANT' named entity 'CHIMERIC' named entity 'VACCINE' named entity 'REVERSE GENETICS' named entity 'ATTENUATION' named entity 'OTHER THAN' named entity 'VIRUS' named entity 'PARTIAL' named entity 'GENE' named entity 'FULLY' named entity 'GENERATED' named entity 'PATTERN' named entity 'HISTOLOGICAL' named entity 'SPIKE' named entity 'AUTOLOGOUS' named entity 'PARENTAL' named entity 'RESULTS' named entity 'PATHOGENESIS' named entity 'LED' named entity 'REPLACING' named entity 'IN S' named entity 'INDUSTRIES' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'INCLUDING' named entity 'STUDY' named entity 'ECONOMIC' named entity 'MUTATIONS' named entity 'VIRULENT' named entity 'EXHIBITING' named entity 'DATA' named entity 'STRAINS' named entity 'ATTENUATED' named entity 'SYSTEM' named entity 'LIVE ATTENUATED VACCINE' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS'

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