About: Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2

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About: Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2
Creator	Liu, Lei Tan, Li Wang, Qiongshu Ding, Yinjuan Kou, Guomei Liu, Wanbing Ni, Wenxu Tang, Shi Wu, Wanlei Zheng, Shangen Zheng, Yaqiong Xiong, Zhou
Source	MedRxiv
abstract	Background: At present, PCR-based nucleic acid detection cannot meet the demands for coronavirus infectious disease (COVID-19) diagnosis. Methods: 214 confirmed COVID-19 patients who were hospitalized in the General Hospital of Central Theater Command of the People's Liberation Army between January 18 and February 26, 2020, were recruited. Two Enzyme-Linked Immunosorbent Assay (ELISA) kits based on recombinant SARS-CoV-2 nucleocapsid protein (rN) and spike protein (rS) were used for detecting IgM and IgG antibodies, and their diagnostic feasibility was evaluated. Results: Among the 214 patients, 146 (68.2%) and 150 (70.1%) were successfully diagnosed with the rN-based IgM and IgG ELISAs, respectively; 165 (77.1%) and 159 (74.3%) were successfully diagnosed with the rS-based IgM and IgG ELISAs, respectively. The positive rates of the rN-based and rS-based ELISAs for antibody (IgM and/or IgG) detection were 80.4% and 82.2%, respectively. The sensitivity of the rS-based ELISA for IgM detection was significantly higher than that of the rN-based ELISA. We observed an increase in the positive rate for IgM and IgG with an increasing number of days post-disease onset (d.p.o.), but the positive rate of IgM dropped after 35 d.p.o. The positive rate of rN-based and rS-based IgM and IgG ELISAs was less than 60% during the early stage of the illness 0-10 d.p.o., and that of IgM and IgG was obviously increased after 10 d.p.o. Conclusions: ELISA has a high sensitivity, especially for the detection of serum samples from patients after 10 d.p.o, it can be an important supplementary method for COVID-19 diagnosis.
has issue date	2020-03-20(xsd:dateTime)
bibo:doi	10.1101/2020.03.16.20035014
has license	medrxiv
sha1sum (hex)	43102c2a4af46ae690f823d77401913f113f092d
schema:url	https://doi.org/10.1101/2020.03.16.20035014
resource representing a document's title	Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2
resource representing a document's body	covid:43102c2a4af46ae690f823d77401913f113f092d#body_text
is schema:about of	named entity 'acid' named entity 'coronavirus' named entity 'nucleic acid detection' named entity 'COVID' named entity 'Nucleocapsid' named entity 'ELISAs' named entity 'serologic' named entity 'ELISAs' named entity 'SARS-CoV-2' named entity 'antibodies' named entity 'd.p.o.' named entity 'IgG' named entity 'serum' named entity 'preprint' named entity 'IgG' named entity 'COVID' named entity 'preprint' named entity 'medRxiv' named entity 'CC-BY-NC-ND 4.0' named entity 'preprint' named entity 'medRxiv' named entity 'infectious disease' named entity 'peer-reviewed' named entity 'China' named entity 'receptor' named entity 'CC-BY-NC-ND 4.0' named entity 'antibodies' named entity 'protein' named entity 'preprint' named entity 'ELISAs' named entity 'medRxiv' named entity 'IgM' named entity 'CC-BY-NC-ND 4.0' named entity 'preprint' named entity 'preprint' named entity 'RBD' named entity 'protein' named entity 'IgM' named entity 'peer-reviewed' named entity 'IgM' named entity 'medRxiv' named entity 'nucleocapsid' named entity 'incubation' named entity 'Europe' named entity 'peer-reviewed' named entity 'negative control' named entity 'antibodies' named entity 'serum' named entity 'IgM' named entity 'immunoassays' named entity 'peer-reviewed' named entity 'preprint' named entity 'angiotensin-converting enzyme 2' named entity 'structural proteins' named entity 'ACE2' named entity 'SARS-CoV-2' named entity 'immunogenic' named entity 'IgG' named entity 'medRxiv' named entity 'IgG' named entity 'IgG' named entity 'pathogens' named entity 'RBD' named entity 'peer-reviewed' named entity 'medRxiv' named entity 'preprint' named entity 'China' named entity 'coronavirus 2' named entity 'preprint' named entity 'IgG' named entity 'preprint' named entity 'ORFs' named entity 'preprint' named entity 'COVID-19'

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