About: Inhibition of enterovirus 71 replication and viral 3C protease by quercetin

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Inhibition of enterovirus 71 replication and viral 3C protease by quercetin
Creator	Cai, Xiaofeng Du, Canghao Gao, Wa Hu, Kanghong Lv, Shiyun Qin, Jinlan Wei, Yanhong Xi, Caili Yao, Chenguang
Source	Medline; PMC
abstract	BACKGROUND: Enterovirus 71 (EV71) is one of the major causative agents of hand, foot, and mouth disease (HFMD), which is sometimes associated with severe central nervous system disease in children. There is currently no specific medication for EV71 infection. Quercetin, one of the most widely distributed flavonoids in plants, has been demonstrated to inhibit various viral infections. However, investigation of the anti-EV71 mechanism has not been reported to date. METHODS: The anti-EV71 activity of quercetin was evaluated by phenotype screening, determining the cytopathic effect (CPE) and EV71-induced cells apoptosis. The effects on EV71 replication were evaluated further by determining virus yield, viral RNA synthesis and protein expression, respectively. The mechanism of action against EV71 was determined from the effective stage and time-of-addition assays. The possible inhibitory functions of quercetin via viral 2Apro, 3C(pro) or 3D(pol) were tested. The interaction between EV71 3C(pro) and quercetin was predicted and calculated by molecular docking. RESULTS: Quercetin inhibited EV71-mediated cytopathogenic effects, reduced EV71 progeny yields, and prevented EV71-induced apoptosis with low cytotoxicity. Investigation of the underlying mechanism of action revealed that quercetin exhibited a preventive effect against EV71 infection and inhibited viral adsorption. Moreover, quercetin mediated its powerful therapeutic effects primarily by blocking the early post-attachment stage of viral infection. Further experiments demonstrated that quercetin potently inhibited the activity of the EV71 protease, 3C(pro), blocking viral replication, but not the activity of the protease, 2A(pro), or the RNA polymerase, 3D(pol). Modeling of the molecular binding of the 3C(pro)-quercetin complex revealed that quercetin was predicted to insert into the substrate-binding pocket of EV71 3C(pro), blocking substrate recognition and thereby inhibiting EV71 3C(pro) activity. CONCLUSIONS: Quercetin can effectively prevent EV71-induced cell injury with low toxicity to host cells. Quercetin may act in more than one way to deter viral infection, exhibiting some preventive and a powerful therapeutic effect against EV71. Further, quercetin potently inhibits EV71 3C(pro) activity, thereby blocking EV71 replication.
has issue date	2018-07-31(xsd:dateTime)
bibo:doi	10.1186/s12985-018-1023-6
bibo:pmid	30064445
has license	cc-by
sha1sum (hex)	412aab9232d9b674c8ba7519a3c85ae72c2f0b9e
schema:url	https://doi.org/10.1186/s12985-018-1023-6
resource representing a document's title	Inhibition of enterovirus 71 replication and viral 3C protease by quercetin
has PubMed Central identifier	PMC6069798
has PubMed identifier	30064445
schema:publication	Virol J
resource representing a document's body	covid:412aab9232d9b674c8ba7519a3c85ae72c2f0b9e#body_text
is schema:about of	named entity 'Background' named entity 'However' named entity 'ENTEROVIRUS 71' named entity 'QUERCETIN' named entity 'MEDICATION' named entity 'DISTRIBUTED' named entity 'SEVERE' named entity 'CAUSATIVE AGENTS' named entity 'MECHANISM' named entity 'FLAVONOIDS' named entity 'DEMONSTRATED' named entity 'ENTEROVIRUS 71' named entity 'VARIOUS' named entity 'BACKGROUND' named entity 'HAND' named entity 'TO INHIBIT' named entity 'REPLICATION' named entity 'INHIBITION' named entity 'CENTRAL NERVOUS SYSTEM DISEASE' named entity 'PLANTS' named entity 'QUERCETIN' named entity 'PROTEASE' named entity 'DATE' named entity 'VIRAL INFECTIONS' named entity 'SPECIFIC' named entity 'FOOT' named entity 'ONE OF' named entity 'MOUTH DISEASE' named entity 'INFECTION' named entity 'VIRAL' named entity 'REPORTED' named entity 'INVESTIGATION' named entity 'CURRENTLY' named entity 'CHILDREN' named entity 'ASSOCIATED WITH' named entity 'MAJOR' covid:arg/412aab9232d9b674c8ba7519a3c85ae72c2f0b9e named entity 'distributed' named entity 'disease' named entity 'Quercetin' named entity 'severe' named entity 'mouth' named entity 'HFMD' named entity 'Enterovirus 71' named entity 'flavonoids' named entity 'EV71' named entity 'hand, foot, and mouth disease' named entity 'quercetin' named entity 'Lipofectamine' named entity 'quercetin' named entity 'viruses' named entity 'neuronal cells' named entity 'antiviral activity' named entity 'peptide' named entity 'DMEM' named entity 'Rutin' named entity 'viral protein' named entity 'quercetin' named entity 'EV71' named entity 'retroviruses' named entity 'non-toxic' named entity 'EV71' named entity 'fluorescence' named entity 'viral RNA' named entity 'HFMD' named entity 'viral adsorption' named entity 'EV71' named entity 'FBS' named entity 'DENV' named entity 'quercetin' named entity 'UMP' named entity 'EV71' named entity 'X-ray structure'

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