About: Development of a rapid immunochromatographic strip test for the detection of porcine epidemic diarrhea virus specific SIgA in colostrum

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About: Development of a rapid immunochromatographic strip test for the detection of porcine epidemic diarrhea virus specific SIgA in colostrum Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of a rapid immunochromatographic strip test for the detection of porcine epidemic diarrhea virus specific SIgA in colostrum
Creator	Feng, Li Chen, Jianfei Shi, Da Shi, Hongyan Zhang, Xin Cao, Liyan Jing, Zhaoyang Liu, Jianbo Wang, Xiaobo Zhang, Jialin Ji, Zhaoyang Cong, Guangyi Gao, Ran
source	Elsevier; Medline; PMC
abstract	Abstract Porcine epidemic diarrhea virus (PEDV) causes very high mortality in newborn piglets. The mucosal immune system in the gut must eliminate potential pathogens while maintaining a mutually beneficial relationship with the commensal microbiota. Antibodies derived from the secretory immunoglobulin A (SIgA) class, act as the first line of antigen-specific immunity in the gut by recognizing both pathogens and commensals. Therefore, the measurement of SIgA levels is an important index in evaluating PEDV infections and immune status. A simple and rapid method for the detection of PEDV-specific SIgA using an immunochromatographic test strip has been developed; incorporating a colloidal gold-labeled anti-SIgA secretory component (SC) mAb probe for the detection of anti-PEDV-specific SIgA in swine. On the strip, a gold-labeled anti-SIgA SC mAb was applied to a conjugate pad; purified PEDV particles and goat anti-mouse antibodies were blotted onto a nitrocellulose membrane to form the test and control lines, respectively. Results showed that the immunochromatographic test strip had high sensitivity and specificity. When compared with enzyme-linked immunosorbent assay, kappa value suggesting that the strip could be used to detect PEDV specific SIgA in colostrum samples. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. We found that the immunochromatographic test strip was a rapid, sensitive, and reliable method for the identification of PEDV specific SIgA, indicating its suitability for epidemiological surveillance as well as vaccine immunity when studying PEDV.
has issue date	2020-05-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2020.113855
bibo:pmid	32173373
has license	els-covid
sha1sum (hex)	411b38711310416a8e45ba63a0d193a6c991bacf
schema:url	https://doi.org/10.1016/j.jviromet.2020.113855
resource representing a document's title	Development of a rapid immunochromatographic strip test for the detection of porcine epidemic diarrhea virus specific SIgA in colostrum
has PubMed Central identifier	PMC7151449
has PubMed identifier	32173373
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:411b38711310416a8e45ba63a0d193a6c991bacf#body_text
is schema:about of	named entity 'vaccine' named entity 'recognizing' named entity 'samples' named entity 'SIgA' named entity 'commensals' named entity 'immunochromatographic' named entity 'IMMUNE STATUS' named entity 'MICROBIOTA' named entity 'IMPORTANT' named entity 'LEVEL' named entity 'PROFESSIONAL' named entity 'TO DETECT' named entity 'IDENTIFICATION' named entity 'strip' named entity 'conjugate' named entity 'sensitivity' named entity 'specific' named entity 'immunity' named entity 'strip' named entity 'high' named entity 'antibodies' named entity 'probe' named entity 'particles' named entity 'indicating' named entity 'purified' named entity 'levels' named entity 'piglets' named entity 'mAb' named entity 'measurement' named entity 'goat' named entity 'vaccine' named entity 'PEDV' named entity 'commensal' named entity 'PEDV' named entity 'enzyme-linked immunosorbent assay' named entity 'swine' named entity 'immunoglobulin' named entity 'antibodies' named entity 'SIgA' named entity 'SIgA' named entity 'immunochromatographic' named entity 'SIgA' named entity 'colostrum' named entity 'microbiota' named entity 'PEDV' named entity 'SIgA' named entity 'immunochromatographic' named entity 'growth medium' named entity 'control line' named entity 'colostrum' named entity 'SIgA' named entity 'protein' named entity 'colloidal gold' named entity 'IgA' named entity 'open reading frames' named entity 'mucosal surface' named entity 'ascites fluid' named entity 'protein' named entity 'Asian' named entity 'immunization' named entity 'specific pathogen free' named entity 'viruses' named entity 'SIgA' named entity 'antibody' named entity 'mAb' named entity 'colostrum' named entity 'immunochromatographic' named entity 'goat' named entity 'IgG'

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