About: Evaluation of envelope glycoprotein E(rns) of an atypical bovine pestivirus as antigen in a microsphere immunoassay for the detection of antibodies against bovine viral diarrhea virus 1 and atypical bovine pestivirus

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About: Evaluation of envelope glycoprotein E(rns) of an atypical bovine pestivirus as antigen in a microsphere immunoassay for the detection of antibodies against bovine viral diarrhea virus 1 and atypical bovine pestivirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of envelope glycoprotein E(rns) of an atypical bovine pestivirus as antigen in a microsphere immunoassay for the detection of antibodies against bovine viral diarrhea virus 1 and atypical bovine pestivirus
Creator	Belák, Sándor Liu, Lihong Xia, Hongyan Harimoorthy, Rajiv Vijayaraghavan, Balaje
topic	covid:40e8efb2cb002dc7fb4b5a1dc7cb6c18d7e4f450#this
Source	Elsevier; Medline; PMC
abstract	Atypical bovine pestiviruses are related antigenically and phylogenetically to bovine viral diarrhea viruses (BVDV-1 and BVDV-2), and may cause the same clinical manifestations in animals. Glycoprotein E(rns) of an atypical bovine pestivirus Th/04_KhonKaen was produced in a baculovirus expression system and was purified by affinity chromatography. The recombinant E(rns) protein was used as an antigen in a microsphere immunoassay for the detection of antibodies against BVDV-1 and atypical bovine pestivirus. The diagnostic performance of the new method was evaluated by testing a total of 596 serum samples, and the assay was compared with enzyme-linked immunosorbent assay (ELISA). Based on the negative/positive cut-off median fluorescence intensity (MFI) value of 2800, the microsphere immunoassay had a sensitivity of 100% and specificity of 100% compared to ELISA. The immunoassay was able to detect antibodies against both BVDV-1 and the atypical pestivirus. This novel microsphere immunoassay has the potential to be multiplexed for simultaneous detection of antibodies against different bovine pathogens in a high-throughput and economical way.
has issue date	2012-07-02(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2012.06.017
bibo:pmid	22766180
has license	no-cc
sha1sum (hex)	40e8efb2cb002dc7fb4b5a1dc7cb6c18d7e4f450
schema:url	https://doi.org/10.1016/j.jviromet.2012.06.017
resource representing a document's title	Evaluation of envelope glycoprotein E(rns) of an atypical bovine pestivirus as antigen in a microsphere immunoassay for the detection of antibodies against bovine viral diarrhea virus 1 and atypical bovine pestivirus
has PubMed Central identifier	PMC7119539
has PubMed identifier	22766180
schema:publication	J Virol Methods
resource representing a document's body	covid:40e8efb2cb002dc7fb4b5a1dc7cb6c18d7e4f450#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/40e8efb2cb002dc7fb4b5a1dc7cb6c18d7e4f450 proxy:http/ns.inria.fr/covid19/40e8efb2cb002dc7fb4b5a1dc7cb6c18d7e4f450
is schema:about of	named entity 'DETECTION' named entity 'atypical' named entity 'antibodies' named entity 'detection' named entity 'BVDV' named entity 'phylogenetically' named entity 'envelope' named entity 'microsphere' named entity 'detection' named entity 'Evaluation' named entity 'POTENTIAL' named entity 'CUT' named entity 'SENSITIVITY' named entity 'TOTAL' named entity 'USED' named entity 'NOVEL' named entity 'ASSAY' named entity 'NEGATIVE' named entity 'RNS' named entity 'BOVINE VIRAL DIARRHEA' named entity 'BOVINE' named entity 'IMMUNOASSAY' named entity 'ATYPICAL' named entity '100%' named entity 'NEW' named entity 'TO DETECT' named entity 'WAY' named entity 'PURIFIED' named entity 'DETECTION' named entity 'COMPARED' named entity 'RELATED' named entity 'ENVELOPE GLYCOPROTEIN' named entity 'MICROSPHERE' named entity 'DIAGNOSTIC' named entity 'MEDIAN FLUORESCENCE INTENSITY' named entity 'RECOMBINANT' named entity 'PERFORMANCE' named entity 'SERUM' named entity 'PESTIVIRUS' named entity 'METHOD' named entity 'PESTIVIRUSES' named entity 'BVDV-2' named entity 'ANTIGEN' named entity 'PESTIVIRUS' named entity 'BOVINE VIRAL DIARRHEA VIRUS 1' named entity 'ATYPICAL' named entity 'BOVINE' named entity 'ANTIBODIES' named entity 'EVALUATION' named entity 'IMMUNOASSAY' named entity 'PROTEIN ' named entity 'AFFINITY CHROMATOGRAPHY' named entity 'CAUSE' named entity 'SAMPLES' named entity 'HIGH' named entity 'CLINICAL MANIFESTATIONS' named entity 'VIRUSES' named entity 'ANTIGEN' named entity 'BVDV-1' named entity 'GLYCOPROTEIN' named entity 'SPECIFICITY' named entity '2800' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'ANIMALS' named entity 'SIMULTANEOUS' named entity 'ELISA' named entity 'BACULOVIRUS EXPRESSION SYSTEM' named entity 'VALUE' named entity 'MICROSPHERE' named entity 'PATHOGENS' named entity 'EVALUATED' named entity 'DIFFERENT' named entity 'THROUGHPUT' named entity 'RNS'

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