About: Verification and diagnostic evaluation of the RealStar(®) Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0

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About: Verification and diagnostic evaluation of the RealStar(®) Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Verification and diagnostic evaluation of the RealStar(®) Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0
Creator	Alamri, Aref Hess, Markus Alzahrani, Fahd Asiri, Mohammed Bochenek, Gregor Halas, Hussein Hasan, Rami Hecht, Leonie-Sophie Jurado-Jimenez, Angeles Mohammed, Hala
Source	Medline; PMC
abstract	AIM: We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. MATERIAL & METHODS: 33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV orf1a and upE, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. RESULTS: 18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. CONCLUSION: The results suggest, that the RealStar(®) MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.
has issue date	2019-07-02(xsd:dateTime)
bibo:doi	10.2217/fmb-2019-0067
bibo:pmid	31271059
has license	cc-by
sha1sum (hex)	40d0c093a36d66c91a2b8cd24e13c14d7f0c7359
schema:url	https://doi.org/10.2217/fmb-2019-0067
resource representing a document's title	Verification and diagnostic evaluation of the RealStar(®) Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0
has PubMed Central identifier	PMC7079715
has PubMed identifier	31271059
schema:publication	Future microbiology
resource representing a document's body	covid:40d0c093a36d66c91a2b8cd24e13c14d7f0c7359#body_text
is schema:about of	named entity 'This' named entity 'Middle East respiratory syndrome' named entity 'MERS-COV RNA' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'PRE' named entity 'CHARACTERIZED' named entity 'SITES' named entity 'DIAGNOSTIC EVALUATION' named entity 'SUITABLE' named entity 'USING' named entity 'REVERSE TRANSCRIPTION' named entity 'STEP' named entity 'TARGETING' named entity 'REPORT' named entity 'GENE' named entity 'RECOMMENDED' named entity 'RT-PCR' named entity 'SAUDI ARABIA' named entity 'CONCLUSION' named entity 'DIAGNOSTICS' named entity 'PCR' named entity 'METHODS' named entity 'RESULTS' named entity 'REAL-TIME RT-PCR' named entity 'NEGATIVE' named entity 'ACCORDING' named entity 'UPE' named entity 'COLLECTIONS' named entity 'RELIABLE' named entity 'SUGGEST' named entity 'POSITIVE' named entity 'GENE' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'VERIFICATION' named entity 'REVERSE TRANSCRIPTION' named entity 'PATIENT' named entity 'FOLLOWS' named entity 'N GENE' named entity 'TESTED' named entity 'COMBINATION' named entity 'CONSIDERED' named entity 'PCR' named entity 'DIAGNOSTIC' named entity 'DIAGNOSTIC PROCEDURE' named entity 'KIT' named entity 'DIAGNOSTIC EVALUATION' named entity 'SCHEME' named entity 'SAMPLES' named entity 'CONFIRMATORY ASSAY' named entity 'CONFIRMATORY' named entity 'MATERIAL' named entity 'KIT' named entity 'UNCERTAIN' named entity 'RIYADH' named entity 'evaluation' named entity 'assay' named entity 'gene' named entity 'Kit' named entity 'genomic DNA' named entity 'gene' named entity 'Material' named entity 'samples' named entity 'PCR' named entity 'diagnostic evaluation' named entity 'MERS-CoV' named entity 'Middle East respiratory syndrome coronavirus' named entity 'gene' named entity 'diagnostic procedure' named entity 'Middle East respiratory syndrome coronavirus' named entity 'open reading frame' named entity 'epidemiological' named entity 'RT-PCR'

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