About: Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1(-/-) Mice

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About: Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1(-/-) Mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1(-/-) Mice
Creator	Li, Xiangdong Zhao, Min Zhao, Yingze Gao, George Liu, Kefang Zhang, X Lu, Xuancheng Zhang, Hangjie Zhang, Yongli Ma, Wenqiang Zhang, Fuping Gao, X Li, F Liu, G Liu, W Liu, William Lu, Y Ma, H Zhang, Citation Zhang, Y Zhao, K Zhao, M
topic	covid:4090c4a0e8cead64dd9e44a1610fb4f557fefdcf#this
Source	Medline; PMC
abstract	The Zika virus (ZIKV) can induce inflammation in immunoprivileged organs (e.g., the brain and testis), leading to the Guillain-Barré syndrome and damaging the testes. During an infection with the ZIKV, immune cells have been shown to infiltrate into the tissues. However, the cellular mechanisms that define the protection and/or immunopathogenesis of these immune cells during a ZIKV infection are still largely unknown. Herein, we describe methods to evaluate the virus-specific T-cell functionality in these immunoprivileged organs of ZIKV-infected mice. These methods include a) a ZIKV infection and vaccine inoculation in Ifnar1(-/-) mice; b) histopathology, immunofluorescence, and immunohistochemistry assays to detect the virus infection and inflammation in the brain, testes, and spleen; c) the preparation of a tetramer of ZIKV-derived T-cell epitopes; d) the detection of ZIKV-specific T cells in the monocytes isolated from the brain, testes, and spleen. Using these approaches, it is possible to detect the antigen-specific T cells that have infiltrated into the immunoprivileged organs and to evaluate the functions of these T cells during the infection: potential immune protection via virus clearance and/or immunopathogenesis to exacerbate the inflammation. These findings may also help to clarify the contribution of T cells induced by the immunization against ZIKV.
has issue date	2018-10-17(xsd:dateTime)
bibo:doi	10.3791/58110
bibo:pmid	30394402
has license	cc-by-nc-nd
sha1sum (hex)	4090c4a0e8cead64dd9e44a1610fb4f557fefdcf
schema:url	https://doi.org/10.3791/58110
resource representing a document's title	Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1(-/-) Mice
has PubMed Central identifier	PMC6235543
has PubMed identifier	30394402
schema:publication	J Vis Exp
resource representing a document's body	covid:4090c4a0e8cead64dd9e44a1610fb4f557fefdcf#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/4090c4a0e8cead64dd9e44a1610fb4f557fefdcf
is schema:about of	named entity 'organs' named entity 'assays' named entity 'testes' named entity 'virus-specific' named entity 'isolated' named entity 'Zika virus' named entity 'induced' named entity 'infection' named entity 'T cells' named entity 'testis' named entity 'T cells' named entity 'immunohistochemistry' named entity 'testes' named entity 'inflammation' named entity 'functions' named entity 'spleen' named entity 'immunization' named entity 'antigen' named entity 'virus' named entity 'immunoprivileged organs' named entity 'ZIKV' named entity 'immunofluorescence' named entity 'infection' named entity 'immunization' named entity 'virus' named entity 'immunopathogenesis' named entity 'spleen' named entity 'ZIKV' named entity 'immune cells' named entity 'ZIKV' named entity 'immunoprivileged organs' named entity 'testis' named entity 'functionality' named entity 'mice' named entity 'ZIKV' named entity 'mice' named entity 'fat' named entity 'viral vaccine' named entity 'T-cell' named entity 'refolding' named entity 'infection' named entity 'cellular immune responses' named entity 'infection' named entity 'mice' named entity 'immunopathogenesis' named entity 'immunoprivileged organs' named entity 'immunofluorescence assay' named entity 'immunoprivileged organs' named entity 'epitope' named entity 'flow cytometry' named entity 'ZIKV' named entity 'CD8 +' named entity 'murine' named entity 'intravenous' named entity 'CNS' named entity 'correlation' named entity 'murine models' named entity 'CD8 +'

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