About: Effects of disinfection on the molecular detection of porcine epidemic diarrhea virus

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About: Effects of disinfection on the molecular detection of porcine epidemic diarrhea virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Effects of disinfection on the molecular detection of porcine epidemic diarrhea virus
Creator	Wang, Qiuhong Bliss, Nola Premanandan, Christopher Stull, Jason Bowman, Andrew Nelson, Sarah Nolting, Jacqueline
Source	Elsevier; Medline; PMC
abstract	Abstract Routine detection of porcine epidemic diarrhea virus (PEDV) is currently limited to RT-PCR but this test cannot distinguish between viable and inactivated virus. We evaluated the capability of disinfectants to both inactivate PEDV and sufficiently damage viral RNA beyond RT-PCR detection. Five classes of disinfectants (phenol, quaternary ammonium compound, sodium hypochlorite, oxidizing agent, and quaternary ammonium/glutaraldehyde combination) were evaluated in vitro at varying concentrations, both in the presence and absence of swine feces, and at three different temperatures. No infectious PEDV was recovered after treatment with evaluated disinfectants. Additionally, all tested disinfectants except for 0.17% sodium hypochlorite dramatically reduced qRT-PCR values. However, no disinfectants eliminated RT-PCR detection of PEDV across all replicates; although, 0.52%, 1.03% and 2.06% solutions of sodium hypochlorite and 0.5% oxidizing agent did intermittently produce RT-PCR negatives. To simulate field conditions in a second aim, PEDV was applied to pitted aluminum coupons, which were then treated with either 2.06% sodium hypochlorite or 0.5% oxidizing agent. Post-treatment surface swabs of the coupons tested RT-PCR positive but were not infectious to cultured cells or naïve pigs. Ultimately, viable PEDV was not detected following application of each of the tested disinfectants, however in most cases RT-PCR detection of viral RNA remained. RT-PCR detection of PEDV is likely even after disinfection with many commercially available disinfectants.
has issue date	2015-09-30(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2015.05.027
bibo:pmid	26072369
has license	els-covid
sha1sum (hex)	4077dcbaea4612b644d31f5c060a46a919e72d3f
schema:url	https://doi.org/10.1016/j.vetmic.2015.05.027
resource representing a document's title	Effects of disinfection on the molecular detection of porcine epidemic diarrhea virus
has PubMed Central identifier	PMC7127254
has PubMed identifier	26072369
schema:publication	Veterinary Microbiology
resource representing a document's body	covid:4077dcbaea4612b644d31f5c060a46a919e72d3f#body_text
is schema:about of	named entity 'evaluated' named entity 'phenol' named entity 'disinfectants' named entity 'hypochlorite' named entity 'pigs' named entity 'tested' named entity 'hypochlorite' named entity 'tested' named entity 'OF EACH' named entity 'INACTIVATED' named entity 'CLASSES' named entity 'APPLICATION' named entity 'DID' named entity '0.17' named entity 'QUATERNARY AMMONIUM COMPOUND' named entity 'DAMAGE' named entity 'CURRENTLY' named entity 'ALUMINUM' named entity 'CONDITIONS' named entity 'VARYING' named entity 'CASES' named entity 'RT-PCR' named entity 'APPLIED' named entity 'QRT-PCR' covid:arg/4077dcbaea4612b644d31f5c060a46a919e72d3f named entity 'PRODUCE' named entity 'COMBINATION' named entity 'EXCEPT FOR' named entity 'INFECTIOUS' named entity 'BEYOND' named entity 'FECES' named entity 'ACROSS' named entity 'TEMPERATURES' named entity 'CULTURED CELLS' named entity 'SOLUTIONS' named entity 'SWINE' named entity 'CONCENTRATIONS' named entity 'SECOND' named entity 'FIELD' named entity 'PRESENCE' named entity 'EFFECTS' named entity 'DISINFECTANTS' named entity 'TREATED WITH' named entity 'AIM' named entity 'ABSENCE OF' named entity 'EVALUATED' named entity 'RECOVERED' named entity 'QUATERNARY AMMONIUM' named entity 'TEST' named entity 'CAPABILITY' named entity 'AVAILABLE' named entity 'VIRUS' named entity 'LIKELY' named entity 'SURFACE' named entity 'PITTED' named entity 'VIABLE' named entity 'DISINFECTION' named entity 'DETECTION' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'POST-TREATMENT' named entity 'VIRAL RNA' named entity 'ELIMINATED' named entity 'REPLICATES' named entity 'DIFFERENT' named entity 'PIGS' named entity 'SWABS' named entity 'AFTER TREATMENT' named entity 'VALUES' named entity 'PCR POSITIVE' named entity 'NOT DETECTED' named entity 'REDUCED' named entity 'DISINFECTION' named entity 'NEGATIVES' named entity 'LIMITED'

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