About: Detection of novel viruses in porcine fecal samples from China

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About: Detection of novel viruses in porcine fecal samples from China Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of novel viruses in porcine fecal samples from China
Creator	Duan, Zhao-Jun Li, Jin-Song Yu, Jie-Mei Ao, Yuan-Yun
topic	covid:40644eccdb08138f90003edef312e6bdc865dab5#this
Source	Medline; PMC
abstract	BACKGROUND: Pigs are well known source of human infectious disease. To better understand the spectrum of viruses present in pigs, we utilized the 454 Life Sciences GS-FLX high-throughput sequencing platform to sequence stool samples from healthy pigs. FINDINGS: Total nucleic acid was extracted from stool samples of healthy piglets and randomly amplified. The amplified materials were pooled and processed using a high-throughput pyrosequencing technique. The raw sequences were deconvoluted on the basis of the barcode and then processed through a standardized bioinformatics pipeline. The unique reads (348, 70 and 13) had limited similarity to known astroviruses, bocaviruses and parechoviruses. Specific primers were synthesized to assess the prevalence of the viruses in healthy piglets. Our results indicate extremely high rates of positivity. CONCLUSIONS: Several novel astroviruses, bocaviruses and Ljungan-like viruses were identified in stool samples from healthy pigs. The rates of isolation for the new viruses were high. The high detection rate, diverse sequences and categories indicate that pigs are well-established reservoirs for and likely sources of different enteric viruses.
has issue date	2013-01-30(xsd:dateTime)
bibo:doi	10.1186/1743-422x-10-39
bibo:pmid	23363449
has license	cc-by
sha1sum (hex)	40644eccdb08138f90003edef312e6bdc865dab5
schema:url	https://doi.org/10.1186/1743-422x-10-39
resource representing a document's title	Detection of novel viruses in porcine fecal samples from China
has PubMed Central identifier	PMC3598965
has PubMed identifier	23363449
schema:publication	Virol J
resource representing a document's body	covid:40644eccdb08138f90003edef312e6bdc865dab5#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/40644eccdb08138f90003edef312e6bdc865dab5
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