About: Porcine Epidemic Diarrhoea Virus Induces Cell-cycle Arrest through the DNA Damage-signalling Pathway

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About: Porcine Epidemic Diarrhoea Virus Induces Cell-cycle Arrest through the DNA Damage-signalling Pathway Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Porcine Epidemic Diarrhoea Virus Induces Cell-cycle Arrest through the DNA Damage-signalling Pathway
Creator	Yang, Liang Dawuli, Yeliboli Hou, Yi-Xuan Jiang, Sheng-Yao Liu, Yuan-Ping Luo, Yi-Ran Yang, Zhi-Biao Zhou, Shu-Ting Zhou, Tian-Xing
Source	Medline; PMC
abstract	INTRODUCTION: Porcine epidemic diarrhoea virus (PEDV) infection causes watery diarrhoea, vomiting, anorexia, and weight loss, especially among neonatal piglets, inflicting on them morbidity and mortality potentially reaching 90%–100%. Despite it being known that certain mammalian cell phases are arrested by PEDV, the mechanisms have not been elucidated, and PEDV pathogenesis is poorly understood. This study determined the effect of an epidemic PEDV strain on cell cycle progression. MATERIAL AND METHODS: We observed the effect of the PEDV SHpd/2012 strain on an infected Vero cell cycle through flow cytometry and Western blot, investigating the interrelationships of cell-cycle arrest, the DNA damage–signalling pathway caused by PEDV and the phosphorylation levels of the key molecules Chk.2 and H2A.X involved upstream and downstream in this pathway. RESULTS: PEDV induced Vero cell-cycle arrest at the G1/G0 phase. The phosphorylation levels of Chk.2 and H2A.X increased with the prolongation of PEDV infection, and no significant cell-cycle arrest was observed after treatment with ATM or Chk.2 inhibitors. The proliferation of PEDV was also inhibited by treatment with ATM or Chk.2 inhibitors. CONCLUSION: PEDV-induced cell-cycle arrest is associated with activation of DNA damage–signalling pathways. Our findings elucidate the molecular basis of PEDV replication and provide evidence to support further evaluation of PEDV pathogenesis.
has issue date	2020-03-24(xsd:dateTime)
bibo:doi	10.2478/jvetres-2020-0024
bibo:pmid	32258796
has license	cc-by-nc-nd
sha1sum (hex)	3fcf1cb78cf4b3258892739d3040bf5d0e0b8011
schema:url	https://doi.org/10.2478/jvetres-2020-0024
resource representing a document's title	Porcine Epidemic Diarrhoea Virus Induces Cell-cycle Arrest through the DNA Damage-signalling Pathway
has PubMed Central identifier	PMC7105999
has PubMed identifier	32258796
schema:publication	J Vet Res
resource representing a document's body	covid:3fcf1cb78cf4b3258892739d3040bf5d0e0b8011#body_text
is schema:about of	named entity 'CERTAIN' named entity 'EFFECT' named entity 'PROLIFERATION' named entity 'TO SUPPORT' named entity 'ON CELL' named entity 'ASSOCIATED WITH' named entity 'H2A.X' named entity 'MOLECULES' named entity 'WATERY DIARRHOEA' named entity 'VERO CELL' named entity 'INTRODUCTION' named entity 'KEY' named entity 'AFTER TREATMENT' named entity 'MORBIDITY AND MORTALITY' named entity 'EVALUATION' named entity 'CELL-CYCLE' named entity 'POORLY' named entity 'DETERMINED' named entity 'PATHWAY' named entity 'LEVELS' named entity 'INDUCED' named entity 'VOMITING' named entity 'INHIBITED' named entity 'DNA DAMAGE' named entity '100%' named entity 'MECHANISMS' named entity 'REPLICATION' named entity 'EPIDEMIC' named entity 'neonatal' named entity 'Vero' named entity 'proliferation' named entity 'provide' named entity 'inhibited' covid:arg/3fcf1cb78cf4b3258892739d3040bf5d0e0b8011 named entity 'FLOW CYTOMETRY' named entity 'NEONATAL' named entity 'INFECTED' named entity 'PORCINE EPIDEMIC DIARRHOEA VIRUS' named entity 'INFECTION' named entity 'SIGNALLING PATHWAY' named entity 'FINDINGS' named entity 'EVIDENCE' named entity 'KNOWN' named entity 'PATHOGENESIS' named entity 'OUR' named entity 'CELL-CYCLE' named entity 'DNA DAMAGE' named entity 'PORCINE EPIDEMIC DIARRHOEA VIRUS' named entity 'SIGNALLING PATHWAY' named entity 'INHIBITORS' named entity 'TREATMENT' named entity 'PEDV' named entity 'CELL CYCLE' named entity '90%' named entity 'UPSTREAM' named entity 'PATHWAYS' named entity 'METHODS' named entity 'MOLECULAR BASIS' named entity 'RESULTS' named entity 'SIGNALLING' named entity 'CAUSES' named entity 'STRAIN' named entity 'REACHING' named entity 'MAMMALIAN CELL' named entity 'DOWNSTREAM' named entity 'CHK' named entity 'UNDERSTOOD' named entity 'PHASE' named entity 'STUDY' named entity 'CONCLUSION' named entity 'WEIGHT LOSS' named entity 'ATM' named entity 'ARREST'

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