About: Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila

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About: Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila
Creator	Mu, Xingjiang Pridgeon, Julia Klesius, Phillip
Source	Elsevier; Medline; PMC
abstract	To determine whether transcriptional levels of channel catfish (Ictalurus punctatus) genes are differentially regulated between a first infection with Aeromonas hydrophila and a re-infection, suppression subtractive hybridization (SSH) was performed in this study using anterior kidney cDNA after the re-infection as tester. Of the 96 clones isolated from the SSH library, 28 unique expressed sequence tags (ESTs) were obtained, of which eight were confirmed to be slightly but significantly (P < 0.05) more up-regulated by the re-infection at 6 h post infection (hpi). Expression kinetics studies at 3, 6, 12, 24, and 48 hpi revealed that the eight ESTs were significantly (P = 0.016) more up-regulated by the first infection, with a major peak at 3 hpi. A total of 96 genes reported in literature to be up-regulated by bacterial infections were selected and subjected to expression analysis at 3 hpi. Of the 96 selected genes, 19 were found to be significantly (P < 0.05) induced by A. hydrophila after the first infection and the re-infection. The 19 genes belonged to the following five main categories: 1) toll-like receptor (TLR2, TLR3, TLR5, TLR21); 2) antimicrobial peptide (NK-lysin type 1, NK-lysin type 2, NK-lysin type 3, cathepsin D, transferrin, hepcidin); 3) cytokine or chemokine (interleukin-1β, interleukin-10, tumor necrosis factor α, chemokine CXCL-10); 4) signaling proteins (cadherin EGF LAG seven-pass G-type receptor 1, very large inducible GTPase 1, arginine deiminase type 2, lymphokine-activated killer T-cell originated protein kinase); 5) lysozyme (lysozyme c). Overall, the total 27 genes (8 ESTs plus the 19 selected genes) were significantly (P < 0.001) more induced by the first infection. Peaked expression of lysozyme c and serum lysozyme activity after the first infection were seen at 24 hpi, whereas that after the re-infection were seen at 12 hpi, suggesting that both innate and adaptive immunity were involved in the defense against the re-infection of A. hydrophila.
has issue date	2013-09-12(xsd:dateTime)
bibo:doi	10.1016/j.fsi.2013.08.027
bibo:pmid	24036330
has license	no-cc
sha1sum (hex)	3f781174b84b70554de1fb3202dcdd555f474bcb
schema:url	https://doi.org/10.1016/j.fsi.2013.08.027
resource representing a document's title	Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila
has PubMed Central identifier	PMC7111657
has PubMed identifier	24036330
schema:publication	Fish Shellfish Immunol
resource representing a document's body	covid:3f781174b84b70554de1fb3202dcdd555f474bcb#body_text
is schema:about of	named entity 'AEROMONAS HYDROPHILA' named entity 'study' named entity 'performed' named entity 'hpi' named entity 'hpi' named entity 'lysozyme' named entity 'kinetics' named entity 'type 1' named entity 'receptor' named entity 'hpi' named entity 'serum' named entity 'cytokine' named entity 'DISTINCT' named entity 'PERFORMED' named entity 'ACTIVATED' named entity 'LIBRARY' named entity 'FOUND' named entity 'REPORTED' named entity 'POST' named entity 'UPREGULATED' named entity 'CHANNEL CATFISH' named entity 'INTERLEUKIN-10' named entity 'UNIQUE' named entity 'CHEMOKINE' named entity 'VERY LARGE INDUCIBLE GTPASE 1' named entity 'BUT' named entity 'INVOLVED' named entity 'LYMPHOKINE' named entity 'CYTOKINE ' named entity 'USING' named entity 'HPI' named entity 'LAG' named entity 'NK-LYSIN' named entity 'TUMOR NECROSIS FACTOR ' named entity 'SLIGHTLY' named entity 'STUDY' named entity 'INFECTION' named entity 'CHANNEL CATFISH' named entity 'SIGNALING' named entity 'OBTAINED' named entity 'CLONES' named entity '016' named entity 'EGF' named entity 'PATTERNS' named entity 'EXPRESSION' named entity 'REVEALS' named entity 'GENES' named entity 'TRANSCRIPTIONAL ANALYSIS' named entity 'KINETICS' named entity 'STUDIES' named entity 'CDNA' named entity '10%' named entity 'KILLER T-CELL' named entity '28P' named entity 'PROTEINS' named entity 'TESTER' named entity 'REINFECTION' named entity 'RECEPTOR' named entity 'ARGININE DEIMINASE' named entity 'PEAK' named entity 'CATHEPSIN D' named entity 'A MAJOR' named entity '0.001' named entity 'TOTAL' named entity 'LYSIN' named entity 'LYSOZYME' named entity 'AEROMONAS HYDROPHILA' named entity 'SSH' named entity 'SUPPRESSION SUBTRACTIVE HYBRIDIZATION' named entity 'TYPE 3' named entity 'REVEALED' named entity 'DETERMINE' named entity 'MAIN' named entity 'TRANSFERRIN' named entity 'LITERATURE' named entity 'BACTERIAL INFECTIONS' named entity 'ADAPTIVE IMMUNITY' named entity 'OVERALL' named entity 'SELECTED'

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