About: Prostaglandin E(2) in a TLR3- and 7/8-agonist-based DC maturation cocktail generates mature, cytokine-producing, migratory DCs but impairs antigen cross-presentation to CD8(+) T cells

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About: Prostaglandin E(2) in a TLR3- and 7/8-agonist-based DC maturation cocktail generates mature, cytokine-producing, migratory DCs but impairs antigen cross-presentation to CD8(+) T cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Prostaglandin E(2) in a TLR3- and 7/8-agonist-based DC maturation cocktail generates mature, cytokine-producing, migratory DCs but impairs antigen cross-presentation to CD8(+) T cells
Creator	Eyrich, Matthias Wölfl, Matthias Sauer, Sascha Gierlich, Philipp Glunz, Amelie Grigoleit, Ulrich Götz, · Hanno Sennholz, · Keupp, Anne Lex, Veronika Lorenz Morper, · Marcu, Ana Rachor, Johannes Schlegel, Paul Technau, Antje
Source	PMC
abstract	Mature dendritic cells (DCs) represent cellular adjuvants for optimal antigen presentation in cancer vaccines. Recently, a combination of prostaglandin E(2) (PGE(2)) with Toll-like receptor agonists (TLR-P) was proposed as a new standard to generate superior cytokine-producing DCs with high migratory capacity. Here, we compare TLR-P DCs with conventional DCs matured only with the proinflammatory cytokines TNFα and IL-1ß (CDCs), focussing on the interaction of resulting DCs with CD8(+) T-cells. TLR-P matured DCs showed elevated expression of activation markers such as CD80 and CD83 compared to CDCs, together with a significantly higher migration capacity. Secretion of IL-6, IL-8, IL-10, and IL-12 was highest after 16 h in TLR-P DCs, and only TLR-P DCs secreted active IL-12p70. TLR-P DCs as well as CDCs successfully primed multifunctional CD8(+) T-cells from naïve precursors specific for the peptide antigens Melan-A, NLGN4X, and PTP with comparable priming efficacy and T-cell receptor avidity. CD8(+) T-cells primed by TLR-P DCs showed significantly elevated expression of the integrin VLA-4 and a trend for higher T-cell numbers after expansion. In contrast, TLR-P DCs displayed a substantially reduced capability to cross-present CMVpp65 protein antigen to pp65-specific T cells, an effect that was dose-dependent on PGE(2) during DC maturation and reproducible with several responder T-cell lines. In conclusion, TLR-P matured DCs might be optimal presenters of antigens not requiring processing such as short peptides. However, PGE(2) seems less favorable for maturation of DCs intended to process and cross-present more complex vaccine antigens such as lysates, proteins or long peptides. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00262-019-02470-1) contains supplementary material, which is available to authorized users.
has issue date	2020-02-25(xsd:dateTime)
bibo:doi	10.1007/s00262-019-02470-1
bibo:pmid	32100075
has license	no-cc
sha1sum (hex)	3ef29f84d7ef41650dc1df3e9c5faf64d12029db
schema:url	https://doi.org/10.1007/s00262-019-02470-1
resource representing a document's title	Prostaglandin E(2) in a TLR3- and 7/8-agonist-based DC maturation cocktail generates mature, cytokine-producing, migratory DCs but impairs antigen cross-presentation to CD8(+) T cells
has PubMed Central identifier	PMC7223547
has PubMed identifier	32100075
schema:publication	Cancer Immunol Immunother
resource representing a document's body	covid:3ef29f84d7ef41650dc1df3e9c5faf64d12029db#body_text
is schema:about of	named entity 'T-cells' named entity 'specific' named entity 'optimal' named entity 'complex' named entity 'represent' named entity 'long' named entity 'T-cell' named entity 'protein' named entity 'DCs' named entity 'cocktail' named entity 'TLR3' named entity 'generates' named entity 'ANTIGENS' named entity 'MATURATION' named entity 'CAPABILITY' named entity 'IL-1' named entity 'DCs' named entity 'primed' named entity 'antigens' named entity 'dendritic cells' named entity 'migration' named entity 'standard' named entity 'integrin' named entity 'cancer vaccines' named entity 'combination' named entity 'PTP' named entity 'activation' named entity 'DCs' named entity 'proteins' named entity 'T cells' named entity 'CD8 +' named entity 'IL-8' named entity 'CD83' named entity 'dose-dependent' named entity 'CD80' named entity 'TLR' named entity 'agonists' named entity 'TLR' named entity 'DCs' named entity 'antigens' named entity 'TLR' named entity 'DCs' named entity 'DCs' named entity 'proinflammatory cytokines' named entity 'TLR' named entity 'NLGN4X' named entity 'IL-6' named entity 'Secretion' named entity 'CDCs' named entity 'PTP' named entity 'TLR3' named entity 'cross-presentation' named entity 'CD8 +' named entity 'proinflammatory cytokines' named entity 'cytokine' named entity 'TLR' named entity 'tyrosine' named entity 'imiquimod' named entity 'DCs' named entity 'organism' named entity 'APC' named entity 'CD3' named entity 'IFNγ' named entity 'cancer vaccine' named entity 'DCs' named entity 'phenotype' named entity 'intracellular'

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