About: Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM

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About: Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM
Creator	Drab, Marek Krasteva, Gabriela Kummer, Wolfgang König, Peter Pfeil, Uwe Planck, Max
Source	Medline; PMC
abstract	BACKGROUND: Caveolae are involved in diverse cellular functions such as signal transduction, cholesterol homeostasis, endo- and transcytosis, and also may serve as entry sites for microorganisms. Hence, their occurrence in epithelium of the airways might be expected but, nonetheless, has not yet been examined. METHODS: Western blotting, real-time quantitative PCR analysis of abraded tracheal epithelium and laser-assisted microdissection combined with subsequent mRNA analysis were used to examine the expression of cav-1 and cav-2, two major caveolar coat proteins, in rat tracheal epithelium. Fluorescence immunohistochemistry was performed to locate caveolae and cav-1 and -2 in the airway epithelium of rats, mice and humans. Electron-microscopic analysis was used for the identification of caveolae. CLSM-FRET analysis determined the interaction of cav-1α and cav-2 in situ. RESULTS: Western blotting and laser-assisted microdissection identified protein and transcripts, respectively, of cav-1 and cav-2 in airway epithelium. Real-time quantitative RT-PCR analysis of abraded tracheal epithelium revealed a higher expression of cav-2 than of cav-1. Immunoreactivities for cav-1 and for cav-2 were co-localized in the cell membrane of the basal cells and basolaterally in the ciliated epithelial cells of large airways of rat and human. However, no labeling for cav-1 or cav-2 was observed in the epithelial cells of small bronchi. Using conventional double-labeling indirect immunofluorescence combined with CLSM-FRET analysis, we detected an association of cav-1α and -2 in epithelial cells. The presence of caveolae was confirmed by electron microscopy. In contrast to human and rat, cav-1-immunoreactivity and caveolae were confined to basal cells in mice. Epithelial caveolae were absent in cav-1-deficient mice, implicating a requirement of this caveolar protein in epithelial caveolae formation. CONCLUSION: These results show that caveolae and caveolins are integral membrane components in basal and ciliated epithelial cells, indicating a crucial role in these cell types. In addition to their physiological role, they may be involved in airway infection.
has issue date	2006-08-11(xsd:dateTime)
bibo:doi	10.1186/1465-9921-7-108
bibo:pmid	16904002
has license	cc-by
sha1sum (hex)	3ebb8d173e6297001673ec3ce3bb14e5c1cb9715
schema:url	https://doi.org/10.1186/1465-9921-7-108
resource representing a document's title	Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM
has PubMed Central identifier	PMC1563466
has PubMed identifier	16904002
schema:publication	Respir Res
resource representing a document's body	covid:3ebb8d173e6297001673ec3ce3bb14e5c1cb9715#body_text
is schema:about of	named entity 'TRANSCYTOSIS' named entity 'CHOLESTEROL HOMEOSTASIS' named entity 'AIRWAYS' named entity 'EXAMINED' named entity 'EXPECTED' named entity 'DETECTED' named entity 'ASSOCIATION' named entity 'AIRWAY EPITHELIUM' named entity 'BACKGROUND' named entity 'ENDO' named entity 'SERVE' named entity 'DIVERSE' named entity 'MIGHT BE' named entity 'OCCURRENCE' named entity 'FUNCTIONS' named entity 'EPITHELIUM' named entity 'RESEARCH' covid:arg/3ebb8d173e6297001673ec3ce3bb14e5c1cb9715 named entity 'CAVEOLIN-1' named entity 'EXPRESSION' named entity 'IN SITU' named entity 'FRET' named entity 'RESPIRATORY' named entity 'BUT' named entity 'CELLULAR' named entity 'SITES' named entity 'CAVEOLAE' named entity 'ENTRY' named entity 'THEIR' named entity 'INVOLVED' named entity 'CLSM' named entity 'SIGNAL TRANSDUCTION' named entity 'MICROORGANISMS' named entity 'signal transduction' named entity 'cellular functions' named entity 'Caveolin-1' named entity 'protein' named entity 'Roche Diagnostics' named entity 'tracheal' named entity 'indirect immunofluorescence' named entity 'homogenates' named entity 'molecular weight' named entity 'PBS' named entity 'homogenized' named entity 'IgG' named entity 'cell type' named entity 'cell types' named entity 'confocal laser scanning microscopy' named entity 'epithelial layer' named entity 'antibody' named entity 'caveolin' named entity 'Heidelberg, Germany' named entity 'RNA' named entity 'microbial' named entity 'epithelium' named entity 'FRET' named entity 'lung' named entity 'immunoreactivity' named entity 'endothelium' named entity 'plasma membrane' named entity 'developmental cycle' named entity 'incubation' named entity 'fluorescence' named entity 'FRET' named entity 'reagent' named entity 'immunofluorescence' named entity 'rat' named entity 'immunofluorescence' named entity 'Wistar rats' named entity 'cell membrane' named entity 'immunoreactivity' named entity 'photobleached' named entity 'CLSM' named entity '1:400' named entity 'EDTA' named entity 'immunolabeling'

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