About: Evaluation of Glass Wool Filters and Hollow-Fiber Ultrafiltration Concentration Methods for qPCR Detection of Human Adenoviruses and Polyomaviruses in River Water

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About: Evaluation of Glass Wool Filters and Hollow-Fiber Ultrafiltration Concentration Methods for qPCR Detection of Human Adenoviruses and Polyomaviruses in River Water Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of Glass Wool Filters and Hollow-Fiber Ultrafiltration Concentration Methods for qPCR Detection of Human Adenoviruses and Polyomaviruses in River Water
Creator	Ahmed, W Gyawali, P Toze, S
Source	Medline; PMC
abstract	Pathogenic human viruses cause over half of gastroenteritis cases associated with recreational water use worldwide. They are difficult to concentrate from environmental waters due to low numbers and small sizes. Rapid enumeration of viruses by quantitative polymerase chain reaction (qPCR) has the potential to improve water quality analysis and risk assessment. However, capturing and recovering these viruses from environmental water remain formidable barriers to routine use. Here, we compared the recovery efficiencies of human adenoviruses (HAdVs) and human polyomaviruses (HPyVs) from 10-L river water samples seeded with raw human wastewater (100 and 10 mL) using hollow-fiber ultrafiltration (HFUF) and glass wool filter (GWF) methods. The mean recovery efficiencies of HAdVs in river water samples through HFUF were 36 and 86 % for 100 and 10 mL of seeded human wastewater, respectively. In contrast, the estimated mean recovery efficiencies of HAdVs in river water samples through GWF were 1.3 and 3 % for 100 and 10 mL seeded raw human wastewater, respectively. Similar trends were also observed for HPyVs. Recovery efficiencies of HFUF method were significantly higher (P < 0.05) than GWF for both HAdVs and HPyVs. Our results clearly suggest that HFUF would be a preferred method for concentrating HAdVs and HPyVs from river water followed by subsequent detection and quantification with PCR/qPCR assays.
has issue date	2016-08-13(xsd:dateTime)
bibo:doi	10.1007/s11270-016-3026-5
bibo:pmid	32214527
has license	no-cc
sha1sum (hex)	3e8a660ca5f97fa24720882a7a1f96a253a2a097
schema:url	https://doi.org/10.1007/s11270-016-3026-5
resource representing a document's title	Evaluation of Glass Wool Filters and Hollow-Fiber Ultrafiltration Concentration Methods for qPCR Detection of Human Adenoviruses and Polyomaviruses in River Water
has PubMed Central identifier	PMC7089043
has PubMed identifier	32214527
schema:publication	Water Air Soil Pollut
resource representing a document's body	covid:3e8a660ca5f97fa24720882a7a1f96a253a2a097#body_text
is schema:about of	named entity 'samples' named entity 'risk assessment' named entity 'capturing' named entity 'estimated' named entity 'raw' named entity 'Ultrafiltration' named entity 'Methods' named entity 'ULTRAFILTRATION' named entity 'DETECTION' named entity 'ADENOVIRUSES' named entity 'EVALUATION' named entity 'FILTERS' named entity 'ANALYSIS' named entity 'ROUTINE' named entity 'MEAN' named entity 'CAUSE' named entity 'PATHOGENIC' named entity 'SMALL' named entity 'WATER USE' named entity '100' named entity 'CASES' named entity 'SIZES' named entity 'RISK ASSESSMENT' named entity '10-L' named entity 'ENUMERATION' named entity 'WORLDWIDE' named entity 'ASSOCIATED WITH' named entity '28P' named entity 'SAMPLES' named entity 'HERE' named entity 'RIVER WATER' named entity 'HUMAN' named entity '1.3' named entity 'GASTROENTERITIS' named entity 'WATERS' named entity 'QUANTITATIVE POLYMERASE CHAIN REACTION' named entity 'CONCENTRATE' named entity 'METHOD' named entity 'THESE' named entity '0.05' named entity 'WATER QUALITY' named entity 'QPCR' named entity 'HUMAN' covid:arg/3e8a660ca5f97fa24720882a7a1f96a253a2a097 named entity 'HOLLOW' named entity 'USE' named entity 'RECREATIONAL' named entity 'RECOVERING' named entity 'SIMILAR' named entity 'METHODS' named entity 'HIGHER' named entity 'ESTIMATED' named entity 'RAW' named entity 'CONCENTRATION' named entity 'USING' named entity 'EFFICIENCIES' named entity 'FIBER' named entity 'GLASS WOOL' named entity 'FIBER' named entity 'RIVER WATER' named entity 'GLASS WOOL' named entity 'POLYOMAVIRUSES' named entity 'WASTEWATER' named entity 'TRENDS' named entity 'OBSERVED' named entity 'NUMBERS' named entity 'POLYOMAVIRUSES' named entity 'FILTER' named entity 'ENVIRONMENTAL' named entity 'METHODS' named entity 'ENVIRONMENTAL WATER' named entity 'HOLLOW' named entity 'LOW' named entity 'CONTRAST' named entity 'VIRUSES' named entity 'POTENTIAL' named entity 'DUE TO' named entity '28100' named entity 'RAPID'

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