About: Evaluation of Fast Technology Analysis (FTA) Cards as an improved method for specimen collection and shipment targeting viruses associated with Bovine Respiratory Disease Complex

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About: Evaluation of Fast Technology Analysis (FTA) Cards as an improved method for specimen collection and shipment targeting viruses associated with Bovine Respiratory Disease Complex Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of Fast Technology Analysis (FTA) Cards as an improved method for specimen collection and shipment targeting viruses associated with Bovine Respiratory Disease Complex
Creator	Chigerwe, Munashe Liang, Xiao Crossley, Beate Hietala, Sharon
Source	Elsevier; Medline; PMC
abstract	Abstract In order to improve the analytic quality of respiratory specimens collected from cattle for nucleic acid-based diagnosis, a study was undertaken to verify realtime PCR efficiency of specimens collected and stabilized on FTA Cards™, filter paper which is treated chemically. Nucleic acids collected using FTA Cards without the need for a cold-chain or special liquid media handling provided realtime PCR results consistent (96.8% agreement, kappa 0.923 [95% CI=0.89–0.96]) with the same specimens collected using traditional viral transport media and shipped on ice using the U.S. Department of Transportation mandated liquid handling requirements. Nucleic acid stabilization on FTA Cards was evaluated over a temperature range (−27°C to +46°C) for up to 14 days to mimic environmental conditions for diagnostic sample handling between collection and processing in a routine veterinary laboratory. No significant difference (P ≥0.05) was observed in realtime PCR cycle threshold values over the temperature range and time storage conditions for Bovine Viral Diarrhea virus, Bovine Respiratory Syncytial virus, Bovine Coronavirus, and Bovine Herpesvirus I. The four viruses evaluated in the study are associated with Bovine Respiratory Disease Complex where improvements in ease and reliability of specimen collection and shipping would enhance the diagnostic quality of specimens collected in the field, and ultimately improve diagnostic efficiency.
has issue date	2014-06-15(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2014.02.022
bibo:pmid	24657552
has license	els-covid
sha1sum (hex)	3dd1ef7ad3df5a9c4cce9e16979c345982cbdb21
schema:url	https://doi.org/10.1016/j.jviromet.2014.02.022
resource representing a document's title	Evaluation of Fast Technology Analysis (FTA) Cards as an improved method for specimen collection and shipment targeting viruses associated with Bovine Respiratory Disease Complex
has PubMed Central identifier	PMC7113650
has PubMed identifier	24657552
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:3dd1ef7ad3df5a9c4cce9e16979c345982cbdb21#body_text
is schema:about of	named entity 'collection' named entity 'FAST' named entity 'SPECIMEN COLLECTION' named entity 'BOVINE RESPIRATORY DISEASE COMPLEX' named entity 'ASSOCIATED WITH' named entity 'SHIPMENT' named entity 'EVALUATION' named entity 'TECHNOLOGY' named entity 'TARGETING' named entity 'METHOD' named entity 'VIRUSES' named entity 'IMPROVED' named entity 'ANALYSIS' named entity 'clinical signs' named entity 't-test' named entity 'BCoV' named entity 'bacteria' named entity 'respiratory disease' named entity 'PCR' named entity 'BRD' named entity 'Bovine Viral Diarrhea virus' named entity 'UV damage' named entity 'oxidation' named entity 'RNA' named entity 'BVDV' named entity '95% CI' named entity 'nucleic acids' named entity 'respiratory syncytial virus' named entity 'coronaviridae' named entity 'virus' named entity '8.2' named entity 'Xeno' named entity 'virus' named entity 'Bovine' named entity 'denature' named entity '80/81' named entity 'primer' named entity 'forensic' named entity 'human DNA' named entity 'BVDV' named entity 'virus' named entity 'nucleic acid' named entity 'genus' named entity 'Respiratory Syncytial virus' named entity 'Bovine' named entity '95% CI' named entity 'primer' named entity 'viruses' named entity 'nucleic acid' named entity 'nucleic acid' named entity 'bovine viral diarrhea virus' named entity 'lyse' named entity 'nucleic acids' named entity 'alphaherpesvirinae' named entity 'infectious agent' named entity 'PCR' named entity 'nucleic acids' named entity 'viruses' named entity 'microbial' named entity 'bovine' named entity '95% CI' named entity 'PCR' named entity 'PCR' named entity 'positive test' named entity 'viruses' named entity 'standard deviation' named entity 'inoculated' named entity 'BCoV' named entity 'statistically significant' named entity 'liquid media' named entity 'nucleic acids' named entity 'nucleic acids' named entity 'PCR' named entity 'real-time PCR' named entity 'viruses' named entity 'Bovine Coronavirus' named entity 'veterinary clinic' named entity 'BCoV'

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