About: Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice

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About: Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice
Creator	Carromeu, Cassiano Farinha-Arcieri, Luis Simabuco, Fernando Tamura, Rodrigo Ventura, Armando
Source	Elsevier; Medline; PMC
abstract	Human respiratory syncytial virus (HRSV) is the major pathogen leading to respiratory disease in infants and neonates worldwide. An effective vaccine has not yet been developed against this virus, despite considerable efforts in basic and clinical research. HRSV replication is independent of the nuclear RNA processing constraints, since the virus genes are adapted to the cytoplasmic transcription, a process performed by the viral RNA-dependent RNA polymerase. This study shows that meaningful nuclear RNA polymerase II dependent expression of the HRSV nucleoprotein (N) and phosphoprotein (P) proteins can only be achieved with the optimization of their genes, and that the intracellular localization of N and P proteins changes when they are expressed out of the virus replication context. Immunization tests performed in mice resulted in the induction of humoral immunity using the optimized genes. This result was not observed for the non-optimized genes. In conclusion, optimization is a valuable tool for improving expression of HRSV genes in DNA vaccines.
has issue date	2009-02-04(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2009.01.024
bibo:pmid	19428575
has license	no-cc
sha1sum (hex)	3dc9494e6e5ca39be44c3e234bf1825a0b9368ce
schema:url	https://doi.org/10.1016/j.jviromet.2009.01.024
resource representing a document's title	Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice
has PubMed Central identifier	PMC7119700
has PubMed identifier	19428575
schema:publication	J Virol Methods
resource representing a document's body	covid:3dc9494e6e5ca39be44c3e234bf1825a0b9368ce#body_text
is schema:about of	named entity 'NUCLEOPROTEIN' named entity 'improving' named entity 'optimized' named entity 'cytoplasmic' named entity 'context' named entity 'robust' named entity 'MICE' named entity 'EXPRESSION' named entity 'HUMORAL IMMUNITY' named entity 'PROTEINS' named entity 'NEONATES' named entity 'USING' named entity 'TRANSCRIPTION' named entity 'CYTOPLASMIC' named entity 'PROCESS' named entity 'IMPROVING' named entity 'EXPRESSED' named entity 'CONSIDERABLE' named entity 'NON-' named entity 'REPLICATION' named entity 'NUCLEOPROTEIN' named entity 'TESTS' named entity 'HUMAN RESPIRATORY SYNCYTIAL VIRUS' named entity '28P' named entity 'OPTIMIZATION' named entity 'PHOSPHOPROTEIN' named entity 'OPTIMIZATION' named entity 'CONCLUSION' named entity 'IS A' named entity 'LEADING' named entity 'RESULT' named entity 'INDUCTION' named entity 'WORLDWIDE' named entity 'IMMUNIZATION' named entity 'CLINICAL RESEARCH' named entity 'NOT OBSERVED' named entity 'RESPIRATORY DISEASE' named entity 'DEPENDENT' named entity 'INFANTS' named entity 'INTRACELLULAR LOCALIZATION' named entity 'TOOL' named entity 'STUDY' named entity 'PERFORMED' named entity 'VIRUS REPLICATION' named entity 'GENES' named entity 'CONTEXT' named entity 'EFFECTIVE' named entity 'RNA-DEPENDENT RNA POLYMERASE' named entity 'PATHOGEN' named entity 'PERFORMED BY' named entity 'GENE' named entity 'ROBUST' named entity 'HUMAN CELLS' named entity 'HUMAN RESPIRATORY SYNCYTIAL VIRUS' named entity 'LEADS' named entity 'HUMORAL IMMUNITY' named entity 'PHOSPHOPROTEIN' named entity 'CHANGES' named entity 'INDEPENDENT OF' named entity 'VIRAL RNA' named entity 'DNA VACCINES' named entity 'RNA POLYMERASE II' named entity 'HRSV' named entity 'MAJOR' named entity 'MICE' named entity 'ADAPTED' named entity 'VACCINE' named entity 'VIRUS' named entity 'RNA PROCESSING' named entity 'THEIR' named entity 'BASIC' named entity 'EXPRESSION' named entity 'INDUCTION' named entity 'NUCLEAR RNA' covid:arg/3dc9494e6e5ca39be44c3e234bf1825a0b9368ce named entity 'intracellular' named entity 'virus'

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