About: Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery
Creator	Rothenberg, Eli Tonzi, Peter Yin, Yandong Huang, Tony Lee, Ting Wei, Chelsea
source	PMC
abstract	DNA replication stress is often defined by the slowing or stalling of replication fork progression leading to local or global DNA synthesis inhibition. Failure to resolve replication stress in a timely manner contribute toward cell cycle defects, genome instability and human disease; however, the mechanism for fork recovery remains poorly defined. Here, we show that the translesion DNA polymerase (Pol) kappa, a DinB orthologue, has a unique role in both protecting and restarting stalled replication forks under conditions of nucleotide deprivation. Importantly, Pol kappa-mediated DNA synthesis during hydroxyurea (HU)-dependent fork restart is regulated by both the Fanconi Anemia (FA) pathway and PCNA polyubiquitination. Loss of Pol kappa prevents timely rescue of stalled replication forks, leading to replication-associated genomic instability, and a p53-dependent cell cycle defect. Taken together, our results identify a previously unanticipated role for Pol kappa in promoting DNA synthesis and replication stress recovery at sites of stalled forks.
has issue date	2018-11-13(xsd:dateTime)
bibo:doi	10.7554/elife.41426
bibo:pmid	30422114
has license	cc-by
sha1sum (hex)	3d950119c81a03ac40e3d304f3fee12617a3316c
schema:url	https://doi.org/10.7554/elife.41426
resource representing a document's title	Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery
has PubMed Central identifier	PMC6251625
has PubMed identifier	30422114
resource representing a document's body	covid:3d950119c81a03ac40e3d304f3fee12617a3316c#body_text
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