About: Triple Immunoglobulin Gene Knockout Transchromosomic Cattle: Bovine Lambda Cluster Deletion and Its Effect on Fully Human Polyclonal Antibody Production

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About: Triple Immunoglobulin Gene Knockout Transchromosomic Cattle: Bovine Lambda Cluster Deletion and Its Effect on Fully Human Polyclonal Antibody Production Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Triple Immunoglobulin Gene Knockout Transchromosomic Cattle: Bovine Lambda Cluster Deletion and Its Effect on Fully Human Polyclonal Antibody Production
Creator	Sullivan, Eddie Wu, Hua Kasinathan, Poothappillai Kuroiwa, Yoshimi Matsushita, Hiroaki Sano, Akiko Wang, Zhongde Jiao, Jin-An Co-, Hiroaki Falls, Sioux Health, Sanford Jiao, Eddie Matsushita, Hua South, Dakota Sullivan, Wu, Jin-An
Source	Medline; PMC
abstract	Towards the goal of producing fully human polyclonal antibodies (hpAbs or hIgGs) in transchromosomic (Tc) cattle, we previously reported that Tc cattle carrying a human artificial chromosome (HAC) comprising the entire unrearranged human immunoglobulin (Ig) heavy-chain (hIGH), kappa-chain (hIGK), and lambda-chain (hIGL) germline loci produced physiological levels of hIgGs when both of the bovine immunoglobulin mu heavy-chains, bIGHM and bIGHML1, were homozygously inactivated (bIGHM(−/−), bIGHML1(−/−); double knockouts or DKO). However, because endogenous bovine immunoglobulin light chain loci are still intact, the light chains are produced both from the hIGK and hIGL genomic loci on the HAC and from the endogenous bovine kappa-chain (bIGK) and lambda-chain (bIGL) genomic loci, resulting in the production of fully hIgGs (both Ig heavy-chains and light-chains are of human origin: hIgG/hIgκ or hIgG/hIgλ) and chimeric hIgGs (Ig heavy-chains are of human origin while the Ig light-chains are of bovine origin: hIgG/bIgκ or hIgG/bIgλ). To improve fully hIgG production in Tc cattle, we here report the deletion of the entire bIGL joining (J) and constant (C) gene cluster (bIGLJ1-IGLC1 to bIGLJ5-IGLC5) by employing Cre/loxP mediated site-specific chromosome recombination and the production of triple knockout (bIGHM(−/−), bIGHML1(−/−) and bIGL(−/−); TKO) Tc cattle. We further demonstrate that bIGL cluster deletion greatly improves fully hIgGs production in the sera of TKO Tc cattle, with 51.3% fully hIgGs (hIgG/hIgκ plus hIgG/hIgλ).
has issue date	2014-03-06(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0090383
bibo:pmid	24603704
has license	cc-by
sha1sum (hex)	3ab1d505d3521db73f63ef84163a41fa2f3dbdf6
schema:url	https://doi.org/10.1371/journal.pone.0090383
resource representing a document's title	Triple Immunoglobulin Gene Knockout Transchromosomic Cattle: Bovine Lambda Cluster Deletion and Its Effect on Fully Human Polyclonal Antibody Production
has PubMed Central identifier	PMC3946162
has PubMed identifier	24603704
schema:publication	PLoS One
resource representing a document's body	covid:3ab1d505d3521db73f63ef84163a41fa2f3dbdf6#body_text
is schema:about of	named entity 'cluster' named entity 'loci' named entity 'production' named entity 'endogenous' named entity 'human' named entity 'Lambda' named entity 'BOVINE' covid:arg/3ab1d505d3521db73f63ef84163a41fa2f3dbdf6 named entity 'DELETION' named entity 'KNOCKOUT' named entity 'PRODUCTION' named entity 'BOVINE' named entity 'REPORT' named entity 'HAC' named entity 'SERA' named entity 'DELETION' named entity 'INACTIVATED' named entity 'HUMAN ARTIFICIAL CHROMOSOME' named entity 'HEAVY-CHAIN' named entity 'PREVIOUSLY' named entity 'HEAVY' named entity 'KAPPA' named entity 'GENE CLUSTER' named entity 'DOUBLE' named entity 'GOAL' named entity 'IMMUNOGLOBULIN' named entity 'GERMLINE' named entity 'CHAINS' named entity 'CHIMERIC' named entity 'CARRYING' named entity 'PHYSIOLOGICAL' named entity 'GREATLY' named entity 'SPECIFIC' named entity 'POLYCLONAL ANTIBODY PRODUCTION' named entity 'TRIPLE' named entity 'HUMAN' named entity 'IGLC5' named entity 'GENOMIC' named entity 'INTACT' named entity 'CONSTANT' named entity 'LAMBDA' named entity 'IMPROVES' named entity 'TRIPLE' named entity 'POLYCLONAL ANTIBODIES' named entity 'HERE' named entity 'CHAIN' named entity 'CHROMOSOME' named entity 'ORIGIN' named entity 'HIGGS' named entity 'HUMAN IMMUNOGLOBULIN' named entity 'LIGHT' named entity 'ITS' named entity 'CATTLE' named entity 'TKO' named entity 'IMMUNOGLOBULIN LIGHT CHAIN' named entity 'ENTIRE' named entity 'JOINING' named entity 'LAMBDA' named entity 'IMMUNOGLOBULIN GENE' named entity 'EFFECT' named entity 'PRODUCING' named entity 'LOCI' named entity 'RECOMBINATION' named entity 'CLUSTER' named entity 'HUMAN' named entity 'HIGL'

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