About: Porcine amino peptidase N domain VII has critical role in binding and entry of porcine epidemic diarrhea virus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Porcine amino peptidase N domain VII has critical role in binding and entry of porcine epidemic diarrhea virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Porcine amino peptidase N domain VII has critical role in binding and entry of porcine epidemic diarrhea virus
Creator	Park, Jung-Eun Shin, Hyun-Jin Park, Eui-Soon Rho, Jaerang Yu, Jung-Eun Kamau, Anthony
Source	Elsevier; Medline; PMC
abstract	Abstract Porcine epidemic diarrhea virus (PEDV) infects swine intestinal cells causing enteric disease. Research has shown that the entry into these cells is through porcine aminopeptidase N (pAPN) receptor. To gain insights into mechanisms of PEDV-pAPN interactions, the present study aimed at identifying the domain that is critical for PEDV binding. To this end, NIH3T3 cell lines constitutively expressing pAPN or pAPN mutants were generated. The mutants were; domain VII deletion mutant and domains IV–VI deletion mutant. In the latter, domain VII was linked to the transmembrane segment through domain III. Results showed PEDV infection was restricted to pAPN and pAPN domain VII expressing NIH3T3 cells. Further, reducing PEDV titre 10 fold resulted in 37.8% decrease in foci indicating positive correlation. A time course test at 12, 24, 36, 48 and 60h showed that foci increased 6 fold in the overall time range. Also, PEDV harvested from pAPN or domain VII expressing NIH3T3 cells was induced indirect plaques in Vero cells confirming successful entry and replication. Collectively, our results demonstrate that PEDV recognizes pAPN and that the main interactive point is lodged within domain VII of the pAPN. These findings are important for therapeutic development as well as creating a platform for future studies on PEDV.
has issue date	2017-01-02(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2016.10.004
bibo:pmid	27732876
has license	els-covid
sha1sum (hex)	3a6a31668f42377d6790de692902556be690dab6
schema:url	https://doi.org/10.1016/j.virusres.2016.10.004
resource representing a document's title	Porcine amino peptidase N domain VII has critical role in binding and entry of porcine epidemic diarrhea virus
has PubMed Central identifier	PMC7114530
has PubMed identifier	27732876
schema:publication	Virus Research
resource representing a document's body	covid:3a6a31668f42377d6790de692902556be690dab6#body_text
is schema:about of	named entity 'ENTRY' named entity 'ROLE' named entity 'porcine' named entity 'infects' named entity 'foci' named entity 'titre' named entity 'Vero' named entity 'indicating' named entity 'interactions' named entity 'VII' named entity 'peptidase' named entity 'VII' named entity 'IMPORTANT' named entity 'POINT' named entity 'SEGMENT' named entity 'INDICATING' named entity 'INTERACTIVE' named entity 'SWINE' named entity 'INDIRECT' named entity 'CORRELATION' named entity 'GAIN' named entity 'BINDING' covid:arg/3a6a31668f42377d6790de692902556be690dab6 named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'DISEASE' named entity 'GENERATED' named entity 'PLAQUES' named entity 'VERO CELLS' named entity 'III' named entity 'REDUCING' named entity 'NIH3T3 CELL' named entity 'NIH3T3' named entity 'RECEPTOR' named entity 'ENTRY' named entity 'POSITIVE' named entity 'OVERALL' named entity 'TIME COURSE' named entity 'END' named entity 'CELLS' named entity 'TEST' named entity 'CONFIRMING' named entity 'CAUSING' named entity 'PORCINE' named entity 'STUDIES' named entity 'CREATING' named entity 'OUR' named entity 'INDUCED' named entity 'REPLICATION' named entity 'INFECTION' named entity 'RESULTS' named entity 'RESEARCH' named entity 'THERAPEUTIC' named entity 'FOCI' named entity 'CELL LINES' named entity 'IDENTIFYING' named entity 'FINDINGS' named entity 'INFECTS' named entity 'INTESTINAL' named entity 'MUTANT' named entity 'DECREASE' named entity 'MAIN' named entity 'FOLD' named entity 'N DOMAIN' named entity 'BINDING' named entity 'PORCINE' named entity 'PEPTIDASE N' named entity 'AMINO' named entity 'CRITICAL' named entity 'PLATFORM' named entity 'INTERACTIONS' named entity 'AMINOPEPTIDASE N' named entity 'RESTRICTED' named entity 'MECHANISMS' named entity 'VII' named entity 'SUCCESSFUL' named entity 'INCREASED'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software