About: In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS

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About: In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS
Creator	Jiang, Hualiang Chen, Jing Luo, Haibin Peng, Shuying Shen, Jianhua Shen, Xu Sun, Tao Wang, Yuan Xie, Youhua Yang, Yiming Ye, Fei Li, Guowei Du, Yi Yue, Liduo
Source	Elsevier; Medline; PMC
abstract	Abstract The major biochemical and thermodynamic features of nucelocapsid protein of SARS coronavirus (SARS_NP) were characterized by use of non-denatured gel electrophoresis, size-exclusion chromatographic and surface plasmon resonance (SPR) techniques. The results showed that SARS_NP existed in vitro as oligomer, more probably dimer, as the basic functional unit. This protein shows its maximum conformational stability near pH 9.0, and it seems that its oligomer dissociation and protein unfolding occur simultaneously. Thermal-induced unfolding for SARS_NP was totally irreversible. Both the thermal and chemical denaturant-induced denaturation analyses showed that oligomeric SARS_NP unfolds and refolds through a two-state model, and the electrostatic interactions among the charge groups of SARS_NP made a significant contribution to its conformational stability.
has issue date	2004-12-01(xsd:dateTime)
bibo:doi	10.1016/j.bpc.2004.06.008
bibo:pmid	15501572
has license	els-covid
sha1sum (hex)	3a5991c5ef4b6910b4220151a8978b01291afe84
schema:url	https://doi.org/10.1016/j.bpc.2004.06.008
resource representing a document's title	In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS
has PubMed Central identifier	PMC7116930
has PubMed identifier	15501572
schema:publication	Biophysical Chemistry
resource representing a document's body	covid:3a5991c5ef4b6910b4220151a8978b01291afe84#body_text
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