About: Summary Single-cell sequencing (sc-Seq) is a powerful tool to investigate the molecular signatures of cell types in a complex mixture of cells. A critical step in sc-Seq is preparing a single-cell suspension with a high number of viable cells. Here, we show how to dissect zebrafish telencephalon and how to dissociate it into a single-cell suspension. This is followed by flow cytometry-based sorting to enrich for neural progenitor stem cells. Our technique typically yields 70000 live cells from one zebrafish telencephalon. For complete details on the use and execution of this protocol, please refer to (Cosacak et al., 2019).   Goto Sponge  NotDistinct  Permalink

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  • Summary Single-cell sequencing (sc-Seq) is a powerful tool to investigate the molecular signatures of cell types in a complex mixture of cells. A critical step in sc-Seq is preparing a single-cell suspension with a high number of viable cells. Here, we show how to dissect zebrafish telencephalon and how to dissociate it into a single-cell suspension. This is followed by flow cytometry-based sorting to enrich for neural progenitor stem cells. Our technique typically yields 70000 live cells from one zebrafish telencephalon. For complete details on the use and execution of this protocol, please refer to (Cosacak et al., 2019).
Subject
  • Biotechnology
  • Animal models
  • DNA sequencing
  • Danio
  • Academic publishing
  • Cerebrum
  • Electronic documents
  • Identifiers
  • Index (publishing)
  • Molecular biology techniques
  • Stem cell research
  • Animal models in neuroscience
  • Fish of Bangladesh
  • Fish of India
  • Fish of Pakistan
  • Regenerative biomedicine
  • Taxa named by Francis Buchanan-Hamilton
  • Fish described in 1822
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