About: Development and validation of direct RT-LAMP for SARS-CoV-2

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and validation of direct RT-LAMP for SARS-CoV-2
Creator	Pabbaraju, Kanti Czub, Markus Greninger, Alexander Lisboa, Luiz Mohon, Abu Pillai, Dylan Van Marle, Guido Church, Deirdre Berenger, Byron Doolan, Cody Griener, Thomas Hundt, Jana
Source	MedRxiv
abstract	We have developed a reverse-transcriptase loop mediated amplification (RT-LAMP) method targeting genes encoding the Spike (S) protein and RNA-dependent RNA polymerase (RdRP) of SARS-CoV-2. The LAMP assay achieves the same limit of detection as commonly used RT-PCR protocols based on artificial targets, recombinant Sindbis virus, and clinical samples. Clinical validation of single target LAMP (N=108) showed a positive percent agreement (PPA) of 33/34 (97.1%) and negative percent agreement (NPA) of 73/74 (98.6%) compared to reference RT-PCR. Dual target RT-LAMP achieved a PPA of 11/11 (100%) and NPA 13/13 (100%) when including discrepant samples. The assay can be performed without a formal extraction procedure, with lyophilized reagents that do need cold chain, and is amenable to point-of-care application with visual detection.
has issue date	2020-05-04(xsd:dateTime)
bibo:doi	10.1101/2020.04.29.20075747
has license	medrxiv
sha1sum (hex)	39b8efc748bbaef79d9e450a666da5c29a9f6746
schema:url	https://doi.org/10.1101/2020.04.29.20075747
resource representing a document's title	Development and validation of direct RT-LAMP for SARS-CoV-2
resource representing a document's body	covid:39b8efc748bbaef79d9e450a666da5c29a9f6746#body_text
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