About: Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model

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About: Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model
Creator	Li, Xunliang Ren, Xiaofeng Li, Guangxing Li, Pengchong Cao, Liyan Liu, He Bai, Yunyun Chen, Huijie
Source	Medline; PMC
abstract	Transmissible gastroenteritis (TGE), caused by transmissible gastroenteritis virus (TGEV), is a highly infectious disease in pigs. Vaccination is an effective approach to prevent TGEV infection. Here, we evaluated the potential of TGEV S1 as a DNA vaccine and porcine interleukin (pIL)-12 as an adjuvant in a mouse model. A DNA vaccine was constructed with the TGEV S1 gene to induce immune response in an experimental mouse model; pIL-12 was chosen as the immunological adjuvant within this DNA vaccine. The pVAX1-(TGEV-S1) and pVAX1-(pIL-12) vectors were transfected into BHK-21 cells and expressed in vitro. Experimental mice were separately immunized with each of the recombinant plasmids and controls through the intramuscular route. The lymphocytes isolated from the blood and spleen were analyzed for proliferation, cytotoxic activities, and populations of CD4(+) and CD8(+) cells. The titers of TGEV S1 in an enzyme-linked immunosorbent assay (ELISA) and TGEV neutralizing antibodies and the concentrations of interferon (IFN)-γ and IL-4 were also analyzed in the serum. The plasmids pVAX1-(TGEV-S1) and pVAX1-(pIL-12) could be expressed in BHK-21 cells, and the combination of pVAX1-(TGEV-S1) and pVAX1-(pIL-12) could induce a significant increase in all markers. pIL-12 could act as an immunological adjuvant in the DNA vaccine for TGEV-S1. Furthermore, the DNA vaccine prepared using TGEV-S1 and porcine IL-12 could induce excellent humoral and cellular immune responses.
has issue date	2019-09-02(xsd:dateTime)
bibo:doi	10.1292/jvms.18-0682
bibo:pmid	31474664
has license	cc-by-nc-nd
sha1sum (hex)	39244c86324ed37f0b32222540efeefd2144d458
schema:url	https://doi.org/10.1292/jvms.18-0682
resource representing a document's title	Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model
has PubMed Central identifier	PMC6863717
has PubMed identifier	31474664
schema:publication	J Vet Med Sci
resource representing a document's body	covid:39244c86324ed37f0b32222540efeefd2144d458#body_text
is schema:about of	named entity 'TARGETING' named entity 'ADJUVANT' named entity 'BHK-21' named entity 'ISOLATED' named entity 'HIGHLY' named entity 'DNA VACCINE' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'CELLULAR' named entity 'A MOUSE' named entity 'SPLEEN' named entity 'COMBINATION' named entity 'COULD BE' named entity 'PIGS' named entity 'IMMUNOLOGICAL ADJUVANT' named entity 'ADJUVANT' named entity 'VACCINATION' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'PROLIFERATION' named entity 'IS A' named entity 'GASTROENTERITIS' named entity 'ACTIVITIES' named entity 'IN VITRO' named entity 'VECTORS' named entity 'EXPERIMENTAL' named entity 'EFFECTIVE' named entity 'lymphocytes' named entity 'TGE' named entity 'humoral' named entity 'plasmids' named entity 'serum' named entity 'immunological adjuvant' named entity 'experimental' named entity 'adjuvant' named entity 'Virology' named entity 'model' named entity 'IMMUNE RESPONSES' named entity 'DNA VACCINE' covid:arg/39244c86324ed37f0b32222540efeefd2144d458 named entity 'PIL-12' named entity 'CONSTRUCTED' named entity 'CHOSEN' named entity 'A MOUSE' named entity 'PORCINE' named entity 'IMPROVES' named entity 'EVALUATED' named entity 'BLOOD' named entity 'IMMUNIZED' named entity 'ANALYZED' named entity 'CELLULAR' named entity 'PLASMID' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'SPIKE' named entity 'GENE' named entity 'MOUSE MODEL' named entity 'CONCENTRATIONS' named entity 'APPROACH' named entity 'INTERFERON ' named entity 'TO PREVENT' named entity 'PREPARED' named entity 'POTENTIAL' named entity 'INDUCE' named entity 'IL-12' named entity 'POPULATIONS' named entity 'ACT' named entity 'NEUTRALIZING ANTIBODIES' named entity 'CAUSED BY' named entity 'PORCINE' named entity 'PAPER' named entity 'EXCELLENT' named entity 'SIGNIFICANT' named entity 'INCREASE' named entity 'LYMPHOCYTES' named entity 'IL-12' named entity 'VIROLOGY'

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