About: Porcine respiratory coronavirus (PRCV) potentiates respiratory disease and proinflammatory cytokine production in the lungs upon intratracheal inoculation with lipopolysaccharide (LPS) at 1 day of infection. This study aimed to quantify LPS-binding protein (LBP), CD14 and haptoglobin in the lungs throughout a PRCV infection. LBP and CD14 recognize LPS and enhance its endotoxic activity, whereas haptoglobin dampens it. Gnotobiotic pigs were inoculated intratracheally with PRCV (n = 34) or saline (n = 5) and euthanized 1–15 days post inoculation (DPI). Virus was detected in the lungs from 1 to 9 DPI. Cell-associated CD14 in lung tissue increased up to 15 times throughout the infection, due to an increase in highly CD14(+) monocyte-macrophages from 1 to 12 DPI and CD14(+) type 2 pneumocytes from 7 to 9 DPI. LBP and soluble CD14 levels in bronchoalveolar lavage fluids were elevated from 1–12 DPI, with up to 35- and 4-fold increases, respectively. Haptoglobin levels increased significantly (×4.5) at 7 DPI. In addition, we found that PRCV could sensitize the lungs to LPS throughout the infection, but the response to LPS appeared less enhanced at the end of infection (7 DPI). The marked increases in LBP, CD14 and haptoglobin were not correlated with the extent of the LPS response.

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About: Porcine respiratory coronavirus (PRCV) potentiates respiratory disease and proinflammatory cytokine production in the lungs upon intratracheal inoculation with lipopolysaccharide (LPS) at 1 day of infection. This study aimed to quantify LPS-binding protein (LBP), CD14 and haptoglobin in the lungs throughout a PRCV infection. LBP and CD14 recognize LPS and enhance its endotoxic activity, whereas haptoglobin dampens it. Gnotobiotic pigs were inoculated intratracheally with PRCV (n = 34) or saline (n = 5) and euthanized 1–15 days post inoculation (DPI). Virus was detected in the lungs from 1 to 9 DPI. Cell-associated CD14 in lung tissue increased up to 15 times throughout the infection, due to an increase in highly CD14(+) monocyte-macrophages from 1 to 12 DPI and CD14(+) type 2 pneumocytes from 7 to 9 DPI. LBP and soluble CD14 levels in bronchoalveolar lavage fluids were elevated from 1–12 DPI, with up to 35- and 4-fold increases, respectively. Haptoglobin levels increased significantly (×4.5) at 7 DPI. In addition, we found that PRCV could sensitize the lungs to LPS throughout the infection, but the response to LPS appeared less enhanced at the end of infection (7 DPI). The marked increases in LBP, CD14 and haptoglobin were not correlated with the extent of the LPS response. Goto Sponge NotDistinct Permalink

An Entity of Type : fabio:Abstract, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	abstract
value	Porcine respiratory coronavirus (PRCV) potentiates respiratory disease and proinflammatory cytokine production in the lungs upon intratracheal inoculation with lipopolysaccharide (LPS) at 1 day of infection. This study aimed to quantify LPS-binding protein (LBP), CD14 and haptoglobin in the lungs throughout a PRCV infection. LBP and CD14 recognize LPS and enhance its endotoxic activity, whereas haptoglobin dampens it. Gnotobiotic pigs were inoculated intratracheally with PRCV (n = 34) or saline (n = 5) and euthanized 1–15 days post inoculation (DPI). Virus was detected in the lungs from 1 to 9 DPI. Cell-associated CD14 in lung tissue increased up to 15 times throughout the infection, due to an increase in highly CD14(+) monocyte-macrophages from 1 to 12 DPI and CD14(+) type 2 pneumocytes from 7 to 9 DPI. LBP and soluble CD14 levels in bronchoalveolar lavage fluids were elevated from 1–12 DPI, with up to 35- and 4-fold increases, respectively. Haptoglobin levels increased significantly (×4.5) at 7 DPI. In addition, we found that PRCV could sensitize the lungs to LPS throughout the infection, but the response to LPS appeared less enhanced at the end of infection (7 DPI). The marked increases in LBP, CD14 and haptoglobin were not correlated with the extent of the LPS response.
subject	Virology Bacterial toxins Clusters of differentiation Smallpox vaccines Taxa named by Carl Linnaeus »more»
part of	Effect of porcine respiratory coronavirus infection on lipopolysaccharide recognition proteins and haptoglobin levels in the lungs
is abstract of	Effect of porcine respiratory coronavirus infection on lipopolysaccharide recognition proteins and haptoglobin levels in the lungs
is hasSource of	covid:ann/target/d56350174764f6152d1a1db593dbcd52f6fe5dbf covid:ann/target/7ded0ba6065c5164462328f83aeb9c70ead6fad5 covid:ann/target/8985c9f20459a7827824114d420b0a7d694fc27e covid:ann/target/8bcef90bf45c697665b3bbd32b190b48a301b34a covid:ann/target/f1a7856ef67672e387a9c6107272d2d094b92c1e »more»

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