About: Antagonistic expression of hepatitis C virus and alpha interferon in lymphoid cells during persistent occult infection

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Antagonistic expression of hepatitis C virus and alpha interferon in lymphoid cells during persistent occult infection
Creator	Mercer, S Inglot, M Macparland, S Michalak, T Mulrooney-Cousins, P Pham, T Simon, K Zalewska, M
source	Medline; PMC
abstract	Summary. Detection of residual HCV in individuals with SVR after treatment of CHC can be significantly heightened by analyzing ex vivo mitogen‐activated T and B lymphocytes and applying sensitive nucleic acid amplification assays. However, it remained unknown if synergistic activation of lymphocytes and monocytes would further augment HCV detection, if viral replication becomes universally upregulated in treated cells, and if examining sequential sera and lymphoid cells would improve detection of occult infection. Using paired sera and lymphoid cells collected 1 year apart from 17 individuals with normal liver enzymes for up to 72 months after SVR, it was found that simultaneous activation of lymphocytes and monocytes enhanced identification of silent HCV infection and revealed that in some cases monocytes were the principal immune cell type where HCV persisted. Testing of serial samples further increased detection of occult infection. Ultimately, by combining the above two approaches, all individuals with SVR were found to be silent carriers of HCV. Clonal sequencing revealed HCV variations in sera and lymphoid cells and evolution of viral genomes confirming ongoing virus replication. Surprisingly, similar to those with CHC, naive lymphoid cells from some individuals carried ∼10(3) HCV copies/μg total RNA. HCV loads in naive lymphoid cells predetermined the outcome of ex vivo stimulation with respect to upregulation or inhibition of HCV replication. HCV RNA levels in occult infection were inversely proportional to the expression of IFNα and IFN‐inducible MxA, but not to IFNγ or tumour necrosis factor α in naive and mitogen‐treated lymphoid cells.
has issue date	2007-01-18(xsd:dateTime)
bibo:doi	10.1111/j.1365-2893.2006.00834.x
bibo:pmid	17650287
has license	no-cc
sha1sum (hex)	37fd97242d1180b921ad1471e40c62fc6d049f31
schema:url	https://doi.org/10.1111/j.1365-2893.2006.00834.x
resource representing a document's title	Antagonistic expression of hepatitis C virus and alpha interferon in lymphoid cells during persistent occult infection
has PubMed Central identifier	PMC7166844
has PubMed identifier	17650287
schema:publication	J Viral Hepat
resource representing a document's body	covid:37fd97242d1180b921ad1471e40c62fc6d049f31#body_text
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