About: The use of cells from ANPEP knockout pigs to evaluate the role of aminopeptidase N (APN) as a receptor for porcine deltacoronavirus (PDCoV)

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About: The use of cells from ANPEP knockout pigs to evaluate the role of aminopeptidase N (APN) as a receptor for porcine deltacoronavirus (PDCoV) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The use of cells from ANPEP knockout pigs to evaluate the role of aminopeptidase N (APN) as a receptor for porcine deltacoronavirus (PDCoV)
Creator	Zhang, Jianqiang Petrovan, Vlad Sheahan, Maureen Beaton, Benjamin Cigan, Mark Stoian, Ana Prather, Randall Rowland, Raymond Wells, Kevin Samuel, Melissa Whitworth, Kristin
Source	Elsevier; Medline; PMC
abstract	Abstract The coronaviruses, porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine deltacoronavirus (PDCoV) represent important sources of neonatal diarrhea on pig farms. The requirement for aminopeptidase N (APN) as a receptor for TGEV, but not for PEDV, is well established. In this study, the biological relevance of APN as a receptor for PDCoV was tested by using CRISPR/Cas9 to knockout the APN gene, ANPEP, in pigs. Porcine alveolar macrophages (PAMs) from ANPEP knockout (KO) pigs showed resistance to PDCoV infection. However, lung fibroblast-like cells, derived from the ANPEP KO PAM cultures, supported PDCoV infection to high levels. The results suggest that APN is a receptor for PDCoV in PAMs but is not necessary for infection of lung-derived fibroblast cells. The infection of the ANPEP KO pigs with PDCoV further confirmed that APN is dispensable as a receptor for PDCoV.
has issue date	2020-02-29(xsd:dateTime)
bibo:doi	10.1016/j.virol.2019.12.007
bibo:pmid	32056711
has license	els-covid
sha1sum (hex)	3791bd8502d747fe86d0b291a01943a62cfd6a2d
schema:url	https://doi.org/10.1016/j.virol.2019.12.007
resource representing a document's title	The use of cells from ANPEP knockout pigs to evaluate the role of aminopeptidase N (APN) as a receptor for porcine deltacoronavirus (PDCoV)
has PubMed Central identifier	PMC7112016
has PubMed identifier	32056711
schema:publication	Virology
resource representing a document's body	covid:3791bd8502d747fe86d0b291a01943a62cfd6a2d#body_text
is schema:about of	named entity 'KNOCKOUT' named entity 'USE OF' named entity 'APN' named entity 'NEONATAL DIARRHEA' named entity 'CELLS' named entity 'DERIVED' named entity 'USING' named entity 'TESTED' named entity 'LIKE' named entity 'IS A' named entity 'REPRESENT' named entity 'FARMS' named entity 'PIGS' named entity 'RECEPTOR' named entity 'BUT' named entity 'AMINOPEPTIDASE N' named entity 'ANPEP' named entity 'PORCINE DELTACORONAVIRUS' named entity 'CELLS' named entity 'ROLE' named entity 'REQUIREMENT' named entity 'ANPEP' named entity 'SUPPORTED' named entity 'LEVELS' named entity 'LUNG' named entity 'PIGS' named entity 'EVALUATE' named entity 'LUNG FIBROBLAST' named entity 'PAM' named entity 'NECESSARY' named entity 'KNOCKOUT' named entity 'BIOLOGICAL' named entity 'SOURCES' named entity 'INFECTION' named entity 'AMINOPEPTIDASE N' named entity 'CONFIRMED' named entity 'ALVEOLAR MACROPHAGES' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'FIBROBLAST' named entity 'GENE' named entity 'PIG' named entity 'IMPORTANT' named entity 'STUDY' named entity 'RESISTANCE TO' named entity 'APN' named entity 'SUGGEST' named entity 'PAMS' named entity 'PORCINE DELTACORONAVIRUS' named entity 'RESULTS' named entity 'ESTABLISHED' named entity 'CULTURES' named entity 'CRISPR' named entity 'RECEPTOR' named entity 'PORCINE' named entity 'CORONAVIRUSES' named entity 'HIGH' named entity 'RELEVANCE' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' covid:arg/3791bd8502d747fe86d0b291a01943a62cfd6a2d named entity 'The infection' named entity 'PAMs' named entity 'lung' named entity 'macrophages' named entity 'Porcine' named entity 'transmissible' named entity 'derived' named entity 'neonatal' named entity 'TGEV' named entity 'receptor' named entity 'diarrhea' named entity 'infection'

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